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Series GSE125739 Query DataSets for GSE125739
Status Public on Jan 29, 2019
Title Transcriptome response of Gluconobacter oxydans against five typical aldehyde inhibitors
Organism Gluconobacter oxydans
Experiment type Expression profiling by array
Summary Toxic inhibitory compounds from lignocellulose pretreatment are the major obstacle to achieve high bioconversion efficiency in biorefinery fermentations. This study shows a unique glucose oxidation catalysis of Gluconobacter oxydans with its gluconic acid productivity free of inhibitor disturbance. The microbial experimentations and the transcriptome analysis revealed that both the activity of the membrane-bound glucose dehydrogenase (mGDH) and the transcription level of the genes in periplasmic glucose oxidation respiratory chain of G. oxydans were essentially not affected under the existence of inhibitory compounds. G. oxydans also rapidly converted furan and phenolic aldehyde inhibitors into the less toxic alcohols or acids. The synergy of the robust periplasmic glucose oxidation and the rapid inhibitor conversion of G. oxydans significantly elevated the efficiency of the oxidative fermentation in lignocellulose hydrolysate. The corresponding genes responsible for the conversion of furan and phenolic aldehyde inhibitors were also mined by DNA microarrays. The synergistic mechanism of G. oxydans provided an important option of metabolic modification for enhancing inhibitor tolerance of general fermentation strains.
 
Overall design Two-condition experiment, aldehyde treated vs. untreated Gluconobacter oxydans cells. Biological replicates: 3 control replicates, 3 furfural treated replicates, 3 5-(hydroxymethyl)-2-furaldehyde (HMF) treated replicates, 3 4-hydroxybenaldehyde treated replicates, 3 syringaldehyde treated replicates, 3 vanillin treated replicates.
 
Contributor(s) Bao J, Zhou P
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 28, 2019
Last update date Jan 30, 2019
Contact name Jie Bao
Organization name East China University of Science and Technology
Street address 130 Meilong Road
City Shanghai
ZIP/Postal code 200237
Country China
 
Platforms (1)
GPL26106 Agilent-080012 Gluconobacter_oxydans_621H
Samples (18)
GSM3581249 G. oxydans, 4hr, 0 aldehyde, replicate 1
GSM3581250 G. oxydans, 4hr, 0 aldehyde, replicate 2
GSM3581251 G. oxydans, 4hr, 0 aldehyde, replicate 3
Relations
BioProject PRJNA517435

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125739_RAW.tar 14.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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