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| Status |
Public on Mar 12, 2019 |
| Title |
Expression profiling of Human Muscle-Derived RNAs within the study Lifelong Football Training: effects on autophagy and healthy longevity promotion |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Aging is a physiological process characterized by a progressive decline of biological functions and an increase in destructive processes in cells and organs. Physical activity and exercise positively affects the expression of skeletal muscle markers involved in longevity pathways. Recently, a new mechanism, autophagy, was introduced to the adaptations induced by acute and chronic exercise as responsible of positive metabolic modification and health-longevity promotion. However, the molecular mechanisms regulating autophagy in response to physical activity and exercise are sparsely described. We investigated the long-term adaptations resulting from lifelong recreational football training on the expression of skeletal muscle markers involved in autophagy signaling. We demonstrated that lifelong football training increased the expression of messengers: RAD23A, HSPB6, RAB1B, TRAP1, SIRT2 and HSBPB1, involved in the auto-lysosomal and proteasome-mediated protein degradation machinery; of RPL1, RPL4, RPL36, MRLP37, involved in cellular growth and differentiation processes; of the Bcl-2, HSP70, HSP90, PSMD13 and of the ATG5-ATG12 protein complex, involved in proteasome promotion and autophagy processes in muscle samples from lifelong trained subjects compared to age-matched untrained controls. In conclusion, our results indicated that lifelong football training positively influence exercise-induced autophagy processes and protein quality control in skeletal muscle, thus promoting healthy aging.
The aim of the present research was to investigate, through a differential transcriptomic approach, the effects of lifelong recreational football training on the expression of key markers involved in the autophagy response for the maintenance of protein quality control, related to healthy longevity promotion, in skeletal muscle from VPG compared to elderly untrained control subjects.
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| Overall design |
Total RNA was extracted from the muscle biopsies of veteran football players (VPG) and of healthy age-matched untrained controls (CG). For strong, unbiased results, pooled RNA libraries were produced by evenly pooling six RNA samples, which resulted in three pooled CGs (named CG1, CG2 and CG3) and three pooled VPG libraries (named VPG1, VPG2 and VPG3). The pooled RNA samples underwent microarray assay to determine gene expression profile. In this study, we carried out profiling with GeneChip® Human Transcriptome Array 2.0 (HTA 2.0, Affymetrix, Santa Clara).
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| Contributor(s) |
Mancini A, Vitucci D, Bredsgaard Randers M, Schmidte JF, Hagman M, Rostgaard Andersend T, Imperlini E, Mandola A, Orrù S, Krustrup P, Buono P |
| Citation(s) |
30837897, 34212217 |
| Submission date |
Jan 29, 2019 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Annamaria mancini |
| E-mail(s) |
annamaria.mancini@uniparthenope.it
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| Phone |
0813737924
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| Organization name |
Università Parthenope
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| Department |
DISMEB
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| Street address |
Via Medina, 40
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| City |
Naples |
| ZIP/Postal code |
80133 |
| Country |
Italy |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA517663 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125830_RAW.tar |
131.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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