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| Status |
Public on May 13, 2019 |
| Title |
Comparison of expressed pdxJ variants on global gene expression in E. coli |
| Platform organisms |
Gluconobacter oxydans; Escherichia coli; Corynebacterium glutamicum; Pseudomonas putida KT2440; Bacillus subtilis subsp. subtilis str. 168 |
| Sample organism |
Escherichia coli BW25113 |
| Experiment type |
Expression profiling by array
|
| Summary |
DNA microarray analyses was carried out to identify genes which are differentially expressed in E. coli when mutant variants of pdxJ are expressed that rescue growth of auxotrophic E. coli delta serB and delta serC mutants.
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| Overall design |
Cells were grown in minimal M9-glucose medium. When a culture reached an optical density of 0.5 cells were harvested by centrifugation for RNA preparation. For each comparison pdxJ(11+) / pdxJ(11-) and pdxJ(X3+)/ pdxJ(WT) two independent biological samples grown from separate cultures were used.
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| Contributor(s) |
Rohweder B, Mack C, Polen T, Bott M, Sterner R |
| Citation(s) |
31557000 |
| Submission date |
Feb 07, 2019 |
| Last update date |
May 25, 2020 |
| Contact name |
Tino Polen |
| E-mail(s) |
t.polen@fz-juelich.de
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| Organization name |
Forschungszentrum Jülich GmbH
|
| Department |
IBG-1: Biotechnology
|
| Street address |
Leo Brandt Str.
|
| City |
Juelich |
| State/province |
NRW |
| ZIP/Postal code |
52425 |
| Country |
Germany |
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| Platforms (1) |
| GPL23003 |
Agilent-069318 FZ_IBG1_4plex array (Feature number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA521348 |
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