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| Status |
Public on May 20, 2019 |
| Title |
Suppressor mutations in ribosomal proteins and FliY restore Bacillus subtilis swarming motility in the absence of EF-P [Ribosome Profiling] |
| Organism |
Bacillus subtilis |
| Experiment type |
Other
|
| Summary |
Translation elongation factor P (EF-P) alleviates ribosome pausing at a subset of motifs encoding consecutive proline residues and is required for growth in many organisms. Here we show that Bacillus subtilis EF-P also alleviated ribosome pausing at sequences encoding tandem prolines, and ribosomes paused within several essential genes without a corresponding growth defect in an efp mutant. The B. subtilis efp mutant is instead impaired for flagellar biosynthesis which results in the abrogation of a form of motility called swarming. We isolate swarming suppressors of efp and identify mutations in 8 genes that suppressed the efp mutant swarming defect, many of which encode conserved ribosomal proteins or ribosome-associated factors. One mutation abolished a translational pause site within the flagellar C-ring component FliY to increase flagellar number and restore swarming motility in the absence of EF-P. Our data support a model wherein EF-P-alleviation of ribosome pausing may be particularly important for macromolecular assemblies like the flagellum that require precise protein stoichiometries.
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| Overall design |
4 Bacillus subtilis strains with 3 biological replicates each were analyzed. DK1042 is the wild type and parent strain.
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| Contributor(s) |
Hummels KR, Kearns DB |
| Citation(s) |
31237868 |
| |
| Submission date |
Feb 07, 2019 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Daniel B. Kearns |
| E-mail(s) |
dbkearns@indiana.edu
|
| Organization name |
Indiana University
|
| Department |
Biology
|
| Lab |
Kearns Lab
|
| Street address |
1001 E 3rd St
|
| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405-7005 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24109 |
Illumina NextSeq 500 (Bacillus subtilis) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE126235 |
Suppressor mutations in ribosomal proteins and FliY restore Bacillus subtilis swarming motility in the absence of EF-P. |
|
| Relations |
| BioProject |
PRJNA521364 |
| SRA |
SRP184511 |
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