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Status |
Public on Mar 07, 2019 |
Title |
Small RNA sequencing of extracellular vesicles from MDA-MB-231 cells treated with PBS, docetaxel (DTX), or doxorubicin (DOXO) |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
To identify the extracellular-vesicle-encapsulated miRNAs that are differentially secreted by the MDA-MB-231 metastatic breast cancer cells following treatment with chemotherapy drugs, we profiled the small RNAs (between 17 and 52 nt) isolated from extracellular vesicles by Illumina sequencing. miRNAs that are significantly induced by chemotherapy drugs are identified.
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Overall design |
Extracellular vesicles were collected by ultracentrifugation of the conditioned medium of MDA-MB-231 cells that had been treated with docetaxel (DTX; 4 nM), doxorubicin (DOXO; 125 nM), or PBS for 48 h. RNA was extracted using TRIZOL LS (Thermo Fisher Scientific), and subjected to library construction and Illumina sequencing.
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Contributor(s) |
Wu X, Wang SE |
Citation(s) |
31118200 |
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Submission date |
Feb 11, 2019 |
Last update date |
Mar 27, 2020 |
Contact name |
Xiwei Wu |
E-mail(s) |
xwu@coh.org
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Organization name |
City of Hope National Medical Center
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Department |
Computational and Quantitative Medicine
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Street address |
1500 E. Duarte Rd.
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City |
Duarte |
State/province |
CA |
ZIP/Postal code |
91010 |
Country |
USA |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (3) |
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Relations |
BioProject |
PRJNA521816 |
SRA |
SRP185599 |