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Series GSE126419 Query DataSets for GSE126419
Status Public on Mar 07, 2019
Title Small RNA sequencing of extracellular vesicles from MDA-MB-231 cells treated with PBS, docetaxel (DTX), or doxorubicin (DOXO)
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary To identify the extracellular-vesicle-encapsulated miRNAs that are differentially secreted by the MDA-MB-231 metastatic breast cancer cells following treatment with chemotherapy drugs, we profiled the small RNAs (between 17 and 52 nt) isolated from extracellular vesicles by Illumina sequencing. miRNAs that are significantly induced by chemotherapy drugs are identified.
 
Overall design Extracellular vesicles were collected by ultracentrifugation of the conditioned medium of MDA-MB-231 cells that had been treated with docetaxel (DTX; 4 nM), doxorubicin (DOXO; 125 nM), or PBS for 48 h. RNA was extracted using TRIZOL LS (Thermo Fisher Scientific), and subjected to library construction and Illumina sequencing.
 
Contributor(s) Wu X, Wang SE
Citation(s) 31118200
Submission date Feb 11, 2019
Last update date Mar 27, 2020
Contact name Xiwei Wu
E-mail(s) xwu@coh.org
Organization name City of Hope National Medical Center
Department Computational and Quantitative Medicine
Street address 1500 E. Duarte Rd.
City Duarte
State/province CA
ZIP/Postal code 91010
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (3)
GSM3598111 MDA231-PBS-EV
GSM3598112 MDA231-DTX-EV
GSM3598113 MDA231-DOXO-EV
Relations
BioProject PRJNA521816
SRA SRP185599

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126419_processed_counts.txt.gz 9.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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