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| Status |
Public on Feb 13, 2019 |
| Title |
Host transcriptome sequencing of FACS-sorted human induced pluripotent stem cell-derived brain endothelial cells infected with Neisseria meningitidis |
| Organisms |
Homo sapiens; Neisseria meningitidis MC58 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study investigates host transcriptome changes of infected human iPS-derived brain endothelial cells in response to prolonged Nm infection (24h)
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| Overall design |
Infected cells were sorted and RNA was extracted. mRNA-Enriched Poly(A)-Tailed RNA was generated. Each cDNA library was then sequenced by Illumina to ~25-30 million reads.
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| Contributor(s) |
Gomes SM, Kim BJ, Menzel AA, Metzger M, Unkmeir AS, Förstner KU, Sauerwein TD, Westermann AJ |
| Citation(s) |
31191497 |
| |
| Submission date |
Feb 12, 2019 |
| Last update date |
Jun 24, 2019 |
| Contact name |
Till David Sauerwein |
| Organization name |
ZB MED - Information Centre for Life Sciences
|
| Department |
Data Science and Services
|
| Street address |
Gleueler Str. 60
|
| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL26178 |
Illumina NextSeq 500 (Homo sapiens; Neisseria meningitidis MC58) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA521959 |
| SRA |
SRP185702 |
