|
| Status |
Public on Mar 23, 2020 |
| Title |
AmpliSeq Transcriptome profiling of human B cells from EBV-infected and FK506-treated humanized mice. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
to investigate the effects of FK506 on the course of EBV infection in vivo
|
| |
|
| Overall design |
we reconstituted NOD-scid IL2Rγc−/− HLA-A2 transgenic mice with CD34+ hematopoietic progenitor cells (HPC) from HLA-A2+ human fetal livers. Upon assessment of human immune cell reconstitution in the peripheral blood at three to four-months after HPC injection, mice were infected with EBV (strain B95-8) or mock-infected with PBS. Three weeks post-infection (p.i.), animals were either treated with subcutaneous (s.c.) injections of FK506 (Tacrolimus) every second day or left untreated for a further two weeks.
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| |
|
| Contributor(s) |
Caduff N, McHugh D, Prummer M, Hoffmann L, Raulf F, Rubic-Schneider T, Traggiai E, Kammüller M, Münz C |
| Citation(s) |
32251475 |
| |
| Submission date |
Feb 13, 2019 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Christian Münz |
| E-mail(s) |
muenzc@immunology.uzh.ch
|
| Organization name |
University Of Zurich
|
| Department |
Institute of Experimental Immunology
|
| Lab |
Viral Immunobiology
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
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| Platforms (1) |
|
| Samples (26)
|
|
| Relations |
| BioProject |
PRJNA522254 |
| SRA |
SRP185825 |
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