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Series GSE126578 Query DataSets for GSE126578
Status Public on Mar 28, 2019
Title A heterochromatin-specific RNA export pathway facilitates piRNA production
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Other
Genome binding/occupancy profiling by high throughput sequencing
Summary PIWI-interacting RNAs (piRNAs) guide transposon silencing in animals. The 22-30nt piRNAs are processed in the cytoplasm from long non-coding RNAs. How piRNA precursors, which often lack RNA processing hallmarks of export-competent transcripts, achieve nuclear export is unknown. Here, we uncover the RNA export pathway specific for piRNA precursors in the Drosophila germline. This pathway requires Nxf3-Nxt1, a variant of the hetero-dimeric mRNA export receptor Nxf1-Nxt1. Nxf3 interacts with UAP56, a nuclear RNA helicase essential for mRNA export, and CG13741/Bootlegger, which recruits Nxf3-Nxt1 and UAP56 to heterochromatic piRNA source loci. Upon RNA cargo binding, Nxf3 achieves nuclear export via the exportin Crm1, and accumulates together with Bootlegger in peri-nuclear nuage, suggesting that after export, Nxf3-Bootlegger delivers precursor transcripts to the piRNA processing sites. Our findings indicate that the piRNA pathway bypasses nuclear RNA surveillance systems to achieve export of heterochromatic, unprocessed transcripts to the cytoplasm, a strategy also exploited by retroviruses.
 
Overall design This study characterizes an RNA export pathway that drives expression of small RNAs (piRNAs) from heterochromatin in Drosophila
 
Contributor(s) Andersen PR, Brennecke J, ElMagrabhy M, Meixner K, Lendl T, Puehringer F, Tirian L
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Submission date Feb 14, 2019
Last update date Mar 30, 2019
Contact name Peter Andersen
E-mail(s) pra@mbg.au.dk
Organization name Aarhus University
Department MBG
Lab Peter Andersen Lab
Street address Universitetsbyen 83
City Aarhus
State/province Denmark
ZIP/Postal code 8000
Country Denmark
 
Platforms (2)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
GPL22106 NextSeq 550 (Drosophila melanogaster)
Samples (44)
GSM3608062 small RNA sequencing from w1118 (wildtype) ovaries, replicate 1
GSM3608063 small RNA sequencing from w1118 (wildtype) ovaries, replicate 2
GSM3608064 small RNA sequencing from rhino knock-out ovaries, replicate 1
Relations
BioProject PRJNA522430
SRA SRP185911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126578_RAW.tar 2.5 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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