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| Status |
Public on Jun 24, 2019 |
| Title |
IFNα-induced gene expression in immortalized pig-tailed macaque CD4+ lymphocytes |
| Organism |
Macaca nemestrina |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to identify IFNα-induced host factor(s) that might inhibit replication of unadapted chimeric SIV/HIV-1 viruses (SHIVs), we measured IFNα-induced gene expression in immortalized pig-tailed macaque (Ptm) CD4+ lymphocytes. Triplicate cultures of Ptm lymphocytes were left untreated or treated with 1000 U/ml of IFNα. Twenty-four hours later, RNA was isolated, and RNA-seq libraries were prepared for sequencing. A total of 198 genes were found to be significantly differentially expressed (|logFC| ≥ 0.585 & FDR 5%) upon IFNα treatment. 147/198 genes were found to be significantly upregulated and 51/198 genes were found to be significantly downregulated regulated upon IFNα treatment.
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| Overall design |
IFNα-induced gene expression in immortalized pig-tailed macaque CD4+ lymphocytes left untreated or treated with 1000 U/ml of IFNα.
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| Contributor(s) |
Sharma A, Overbaugh J |
| Citation missing |
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| |
| Submission date |
Feb 14, 2019 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Amit Sharma |
| E-mail(s) |
asharma2@fredhutch.org
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| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Human Biology
|
| Lab |
Julie Overbaugh
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| Street address |
1100 Fairview Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL19597 |
Illumina HiSeq 2500 (Macaca nemestrina) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA522470 |
| SRA |
SRP185942 |
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