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| Status |
Public on Feb 16, 2019 |
| Title |
Genome-wide map of viral transcription factor ORF34 binding in Kaposi's Sarcoma Herpesvirus (KSHV) |
| Organisms |
Homo sapiens; Human gammaherpesvirus 8 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the mapping of the genome-wide binding sites of a central component of a viral transcription complex from KSHV. We find that ORF34 binds at several locations on the viral genome but observed no detectable binding to the host genome. A majority of binding sites on the viral genome were upstream of known transcription start sites of mapped genes.
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| Overall design |
Chromatin for immuno-precipitation was prepared from iSLK cells latently infected with HA-ORF34 KSHV and reactivated with doxycycline and sodium butyrate for 48 h.
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| Contributor(s) |
Nandakumar D, Glaunsinger B |
| Citation(s) |
31095645 |
| |
| Submission date |
Feb 14, 2019 |
| Last update date |
May 20, 2019 |
| Contact name |
Britt Glaunsinger |
| Organization name |
UC Berkeley
|
| Street address |
565 Li Ka Shing Center
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL25719 |
Illumina HiSeq 4000 (Homo sapiens; Human gammaherpesvirus 8) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE126603 |
An integrative approach identifies direct targets of the late viral transcription complex and an expanded promoter recognition motif in Kaposi’s sarcoma-associated herpesvirus |
|
| Relations |
| BioProject |
PRJNA522742 |
| SRA |
SRP185998 |
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