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| Status |
Public on May 08, 2019 |
| Title |
Measuring the influence of RNA binding proteins on A-to-I RNA editing in the Drosophila brain |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A-to-I RNA editing levels differ across tissues and cell types, but regulators of the editing process are largely unknown. We used RNA-seq on whole fly brains with different RNA binding proteins knocked down to test for A-to-I RNA editing level differences between controls and knockdowns.
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| Overall design |
To screen for editing regulators in the Drosophila brain, we crossed a pan-neuronal Gal4 driver, C155-Gal4, to different UAS-shRNA lines targeting individual RNA binding proteins, extracted RNA and made RNA-seq libraries. We sequenced four total replicates of shGFP controls and two replicates of all RNA binding protein knockdowns.
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| Contributor(s) |
Sapiro AL, Qiao H, Ni J, Li JB |
| Citation(s) |
32433963 |
| |
| Submission date |
Feb 15, 2019 |
| Last update date |
Jun 29, 2020 |
| Contact name |
Jin Billy Li |
| E-mail(s) |
jin.billy.li@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Street address |
300 Pasteur Dr, Alway M341
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (48)
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| This SubSeries is part of SuperSeries: |
| GSE126631 |
Zinc finger RNA binding protein Zn72D regulates ADAR-mediated RNA editing in neurons |
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| Relations |
| BioProject |
PRJNA522773 |
| SRA |
SRP186005 |
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