|
| Status |
Public on May 20, 2019 |
| Title |
Inducible histone K-to-M mutations are dynamic tools to probe the physiological role of site-specific histone methylation in vitro and in vivo [ATAC] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Here, we developed mouse models expressing inducible lysine to methionine mutants of histone H3, which function as dominant negative inhibitors of methylation at their respective sites. Upon induction of H3K9M or H3K36M, we observed potent differentiation defects in stem cells and regenerative tissues. This work covers RNA-seq and ATAC-seq in this model system.
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| |
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| Overall design |
ATAC-seq in blood stem and progenitor cells as well as embryoid bodies.
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| |
|
| Contributor(s) |
Brumbaugh J, Ji F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 20, 2019 |
| Last update date |
May 21, 2019 |
| Contact name |
Justin Brumbaugh |
| Organization name |
University of Colorado Boulder
|
| Department |
Molecular, Cellular, and Developmental Biology
|
| Street address |
347 UCB
|
| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80309 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE126829 |
Inducible histone K-to-M mutations are dynamic tools to probe the physiological role of site-specific histone methylation in vitro and in vivo |
|
| Relations |
| BioProject |
PRJNA523406 |
| SRA |
SRP186400 |
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