NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE126844 Query DataSets for GSE126844
Status Public on Sep 15, 2019
Title Transcriptomic analysis of human extravillous trophoblast cells (HTR8/Svneo) cultured as 3D spheroids and 2D monolayers
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The current objectives are to develop a 3D spheroid model that will allow us to better emulate placental invasion in vitro and to characterize the transcriptomic and functional outcomes.
 
Overall design 200 ng of RNA was processed to assess 20,000+ genes using the Clarion S Human microarray (Thermo Fisher) and GeneChip™ 3000 instrument system (Thermo Fisher), all carried out at the SickKids TCAG Microarray facility. There are four biological replicates per group, and two groups in total (3D vs. 2D). Normalization and output file generation was performed with Affymetrix Gene Expression Console (Thermo) using the Robust Multi-Chip Analysis (RMA) algorithm [RMA-Gene-full].
 
Contributor(s) Wong MK, Wahed M, Shawky SA, Wahed M, Raha S
Citation(s) 31471547
Submission date Feb 21, 2019
Last update date Sep 23, 2019
Contact name Michael Wong
E-mail(s) wongm20@mcmaster.ca
Organization name McMaster University
Street address 1200 Main Street West, Health Sciences Building
City Hamilton
State/province Ontario
ZIP/Postal code L8N 3Z5
Country Canada
 
Platforms (1)
GPL23159 [Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay)
Samples (8)
GSM3615131 2D HTR8 A
GSM3615132 2D HTR8 B
GSM3615133 2D HTR8 C
Relations
BioProject PRJNA523498

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126844_RAW.tar 9.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.