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| Status |
Public on Jan 31, 2021 |
| Title |
Sources of Transcription Variation in Plasmodium falciparum |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by array
|
| Summary |
The parasite strains HB3 and Dd2 were cloned via limiting dilution and then sub-cloned. The uncloned parental line, 4 clones and 4 sub-clones were thawed in two different culture batches using different media lots and RBC donors but were otherwise maintained in identical standard culture conditions and synchronized by 3 rounds of sorbitol synchronization. Total RNA samples were collected at 24 hours post invasion (hpi).
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| Overall design |
The parasite strains HB3 and Dd2 were cloned via limiting dilution and then sub-cloned. The uncloned parental line, 4 clones and 4 sub-clones were thawed in two different culture batches using different media lots and RBC donors but were otherwise maintained in identical standard culture conditions and synchronized by 3 rounds of sorbitol synchronization. Total RNA samples were collected at 24 hours post invasion (hpi).
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| Contributor(s) |
Turnbull LB, Button-Simons KA, Ferdig MT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 21, 2019 |
| Last update date |
Feb 02, 2021 |
| Contact name |
Michael T Ferdig |
| E-mail(s) |
ferdig.1@nd.edu
|
| Organization name |
University of Notre Dame
|
| Department |
Biological Sciences
|
| Lab |
Ferdig lab
|
| Street address |
100 Galvin Life Sciences Center
|
| City |
Notre Dame |
| State/province |
IN |
| ZIP/Postal code |
46556 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL22280 |
Agilent-061786 Pfal_Exome_Array (Gene-level version) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA523587 |
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