|
| Status |
Public on Nov 01, 2019 |
| Title |
RNA-sequencing analysis of colon tissue in a murine EAE model after dietary tryptophan deprivation. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In the experimental autoimmune encephalomyelitis (EAE) model we found that omission of the essential amino acid tryptophan (trp) abrogates central nervous system (CNS) autoimmunity. Impaired encephalitogenic T responses were accompanied by an inflammatory response in the colonic mucosa, as well as a profound shift in the gut microbiota. Here, we performed transcriptional profiling of colon tissue by RNA-sequencing 14 days after disease induction in mice that received a trp-free diet or a matched control group to identify key networks that drive this immunosuppressive effect.
|
| |
|
| Overall design |
Two groups of female C57BL/6J wildtype mice were immunized with myelin oligodendrocyte glycoprotein (MOG)35-55 in Complete Freund’s Adjuvant (CFA) at the age of 7-8 weeks. One group received a trp-free diet from day 0 on until the end of the experiment. Distal colon tissue for RNA-sequencing analysis was collected 14 days post-immunization.
|
| |
|
| Contributor(s) |
Sonner JK, Mishra N, Falk-Paulsen M, Kramer M, Deumelandt K, Wolf L, Rosenstiel P, Platten M |
| Citation(s) |
31653831 |
| |
| Submission date |
Feb 26, 2019 |
| Last update date |
Sep 11, 2020 |
| Contact name |
Neha Mishra |
| E-mail(s) |
n.mishra@ikmb.uni-kiel.de
|
| Organization name |
Institute of Clinical Molecular Biology
|
| Lab |
Cell biology Lab
|
| Street address |
Rosalind-Franklin-Str. 12
|
| City |
Kiel |
| ZIP/Postal code |
24105 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (19)
|
|
| Relations |
| BioProject |
PRJNA524321 |
| SRA |
SRP186885 |
Less...
More...