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| Status |
Public on Apr 30, 2019 |
| Title |
Field pathogenomics of Fusarium head blight reveals pathogen transcriptome differences due to host resistance |
| Organism |
Fusarium graminearum |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Fusarium head blight (FHB), caused by Fusarium graminearum, is a detrimental disease that affects small grains such as wheat around the world. Management of FHB is difficult, and improved methods of surveillance as well as a better understanding of pathogen aggressiveness are needed for improved control. F. graminearum disease severity varies depending on the resistance of the host genotype. In this study, we used the field pathogenomics method to investigate gene expression and population structure of isolates collected from wheat lines of varying resistance levels (susceptible, intermediate, and resistant) as well as an axenic control. Differential gene expression was found among isolates collected from different host genotypes. Candidate gene sets were identified for both F. graminearum infection of specific host genotypes and general infection to wheat. Population structure of isolates from different resistance level sources was the same, with all isolates belonging to the NA1 population.
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| Overall design |
SRA Study: SRP159931, Bioproject: PRJNA486288. Naturally infected head spikelet samples were collected from 3 wheat varieties (resistant, intermediate and susceptible to Fusarium head blight) from 4 different locations in central Illinios, plus an axenic control from a pure culture of F. graminearum. 13 samples total
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| Contributor(s) |
Fall LA, Salazar M, Drnevich J, Holmes JR, Tseng M, Kolb FL, Mideros SX |
| Citation(s) |
31112486 |
| BioProject |
PRJNA486288 |
| Submission date |
Feb 27, 2019 |
| Last update date |
Jul 30, 2019 |
| Contact name |
Santiago Xavier Mideros |
| E-mail(s) |
santiagomideros@gmail.com
|
| Phone |
6073398711
|
| Organization name |
University of Illinois at Urbana Champaign
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| Department |
Crop Sciences
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| Lab |
Mideros
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| Street address |
AW-101 Turner Hall / 1102 S. Goodwin Av.
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| City |
Urbana |
| State/province |
Illinois |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
| GPL24502 |
Illumina HiSeq 4000 (Fusarium graminearum) |
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| Samples (13)
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| Relations |
| SRA |
SRP159931 |
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