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Series GSE127334 Query DataSets for GSE127334
Status Public on Apr 30, 2019
Title Field pathogenomics of Fusarium head blight reveals pathogen transcriptome differences due to host resistance
Organism Fusarium graminearum
Experiment type Expression profiling by high throughput sequencing
Summary Fusarium head blight (FHB), caused by Fusarium graminearum, is a detrimental disease that affects small grains such as wheat around the world. Management of FHB is difficult, and improved methods of surveillance as well as a better understanding of pathogen aggressiveness are needed for improved control. F. graminearum disease severity varies depending on the resistance of the host genotype. In this study, we used the field pathogenomics method to investigate gene expression and population structure of isolates collected from wheat lines of varying resistance levels (susceptible, intermediate, and resistant) as well as an axenic control. Differential gene expression was found among isolates collected from different host genotypes. Candidate gene sets were identified for both F. graminearum infection of specific host genotypes and general infection to wheat. Population structure of isolates from different resistance level sources was the same, with all isolates belonging to the NA1 population.
Overall design SRA Study: SRP159931, Bioproject: PRJNA486288. Naturally infected head spikelet samples were collected from 3 wheat varieties (resistant, intermediate and susceptible to Fusarium head blight) from 4 different locations in central Illinios, plus an axenic control from a pure culture of F. graminearum. 13 samples total
Contributor(s) Fall LA, Salazar M, Drnevich J, Holmes JR, Tseng M, Kolb FL, Mideros SX
Citation(s) 31112486
BioProject PRJNA486288
Submission date Feb 27, 2019
Last update date Jul 30, 2019
Contact name Santiago Xavier Mideros
Phone 6073398711
Organization name University of Illinois at Urbana Champaign
Department Crop Sciences
Lab Mideros
Street address AW-101 Turner Hall / 1102 S. Goodwin Av.
City Urbana
State/province Illinois
ZIP/Postal code 61801
Country USA
Platforms (1)
GPL24502 Illumina HiSeq 4000 (Fusarium graminearum)
Samples (13)
GSM3634077 Resistant line from location 1
GSM3634078 Resistant line from location 2
GSM3634079 Resistant line from location 3
SRA SRP159931

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127334_RAW.tar 5.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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