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| Status |
Public on Aug 22, 2019 |
| Title |
Next Generation Sequencing Analysis of the Transcriptomes of Wild Type and ALKBH5-/- Peritoneal Macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
By performing RNA sequencing (RNA-seq) analysis on the peritoneal macrophages that derived from ALKBH5-/- mice and littermate mice infected with or without vesicular stomatitis virus (VSV), we want to investigate whether deficiency of ALKBH5 controls viral replication through a more general mechanism such as enhancing innate response, and if not, what's the critical downstream target(s) of ALKBH5. RNA-seq analysis showed that mRNA expression of innate genes remained unchanged or not increased in ALKBH5-deficient cells. While thourgh KEGG pathway enrichment analysis of the RNA-seq data, we found that metabolic pathways were the ones most significantly affected in ALKBH5-deficient macrophages upon VSV infection. These results demonstrated that deficiency of ALKBH5 controls viral replication by significantly changing the metabolism-related genes expression.
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| Overall design |
Transcriptomes profiles of gene expression changes in wild type and ALKBH5-/- peritoneal macrophages infected with or without VSV, two biological replicates.
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| Contributor(s) |
Liu Y |
| Citation(s) |
31439758 |
| |
| Submission date |
Mar 01, 2019 |
| Last update date |
Aug 26, 2019 |
| Contact name |
Yang Liu |
| E-mail(s) |
yliu@immunol.org
|
| Organization name |
Peking Union Medical College&Chinese Academy of Medical Sciences
|
| Department |
Immunology
|
| Street address |
Dongdan Santiao 5
|
| City |
Beijing |
| ZIP/Postal code |
100005 |
| Country |
China |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA525150 |
| SRA |
SRP187301 |
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