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| Status |
Public on Feb 01, 2020 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of E. coli K-12 LE392, P. putida KT2440, and RP4 plasmid Transcriptomes under AgNP and silver ion exposure |
| Organisms |
Escherichia coli K-12; Pseudomonas putida KT2440 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goals of this study are to use Next-generation sequencing (NGS)to detect bacterial mRNA profiles of E. coli K-12 LE392, P. putida KT2440 and IncPα RP4 plasmid in response to 0 and 1 μg/L AgNPs or silver ion for 2 h, in duplicate, using Illumina HiSeq 2500.The NGS QC toolkit (version 2.3.3) was used to treat the raw sequence reads to trim the 3’-end residual adaptors and primers, and the ambiguous characters in the reads were removed. Then, the sequence reads consisting of at least 85% bases were progressively trimmed at the 3’-ends until a quality value ≥ 20 were kept. Downstream analyses were performed using the generated clean reads of no shorter than 75 bp. The clean reads of each sample were aligned to the E. coli reference genome (NC_000913) using SeqAlto (version 0.5). Cufflinks (version 2.2.1) was used to calculate the strand-specific coverage for each gene, and to analyze the differential expression in triplicate bacterial cell cultures. The statistical analyses and visualization were conducted using CummeRbund package in R (http://compbio.mit.edu/cummeRbund/). Gene expression was calculated as fragments per kilobase of a gene per million mapped reads (FPKM, a normalized value generated from the frequency of detection and the length of a given gene.
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| Overall design |
Bacterial mRNA profiles of E. coli K-12 LE392 in response to 0and 1 μg/L AgNPs and silver ion for 2 h, in duplicate, using Illumina HiSeq 2500
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| Contributor(s) |
Lu J, Guo J |
| Citation(s) |
31783256 |
| |
| Submission date |
Mar 13, 2019 |
| Last update date |
May 02, 2020 |
| Contact name |
Jianhua Guo |
| E-mail(s) |
j.guo@awmc.uq.edu.au
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| Organization name |
University of Queensland
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| Department |
Australian Centre for Water and Environmental Biotechnology
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| Street address |
Research Road, Level 4 Gehrmann Building
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| City |
Brisbane |
| ZIP/Postal code |
4072 |
| Country |
Australia |
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| Platforms (1) |
| GPL26298 |
Illumina HiSeq 2500 (Escherichia coli K-12; Pseudomonas putida KT2440) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA526893 |
| SRA |
SRP188351 |
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