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| Status |
Public on Jul 01, 2019 |
| Title |
Histone 3 K9 and K27 acetylation of enhancers in response to thyroid hormone |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Hormone dependent activation of enhancers includes histone hyperacetylation and mediator recruitment. Histone hyperacetylation is often explained by a bimodal switch mod-el, where histone deacetylases (HDACs) disassociates from chromatin and histone acetyl transferases (HATs) are recruited. This model builds on decades of research on steroid re-ceptor regulation of transcription. We have used a genomics approach to study enhancer hyperacetylation by the thyroid hormone receptor (TR) and present a revised model. 1) at poised constitutively TR bound enhancers, HATs occupy chromatin irrespective of thyroid hormone (T3) levels, whereas HDAC occupancy is regulated by T3, suggesting that HDACs functions as a histone acetylation rheostat. 2) at enhancers established in a T3 dependent manner, TR is recruited to chromatin together with HATs. 3) a number of enhancers are hy-peracetylated secondary to TR activation. Collectively, this demonstrates various mechanisms controlling hormone dependent transcription and adds significant details to the otherwise simple bimodal switch model.
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| Overall design |
ChIP-seq was performed in replicates from a pool of four individual ChIPs from livers of four individual mice. Some ChIP-seq's were not performed in replicates. The RNA-seq was performed on four indepedent biological replicates.
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| Contributor(s) |
Grøntved L, Præstholm SM |
| Citation missing |
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| Submission date |
Mar 19, 2019 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Lars Grøntved |
| E-mail(s) |
larsgr@bmb.sdu.dk
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| Phone |
+45 24 60 14 06
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| Organization name |
University of Southern Denmark
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| Department |
Biochemistry and Molecular Biology
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| Street address |
Campusvej 55
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| City |
Odense |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
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| Platforms (2) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (56)
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| Relations |
| BioProject |
PRJNA528027 |
| SRA |
SRP188846 |
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