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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 24, 2019 |
| Title |
ERG controls B-cell development by promoting Igh V-to-DJ recombination [microarray] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
B-cell development is dependent on the coordinated expression and cooperation of several transcription factors. Here, we show that the transcription factor ETS Related Gene (ERG) is crucial for normal B-cell development, and that its deletion results in a substantial loss of bone marrow B-cell progenitors and peripheral B-cells as well as a skewing of splenic B-cell populations. We found that ERG-deficient B-lineage cells exhibited an early developmental block, at the pre-B cell stage, and proliferated less. The cells failed to express the immunoglobulin heavy chain due to inefficient V-to-DJ recombination and cells that managed to undergo recombination displayed a strong bias against incorporation of distal V gene segments. Furthermore, antisense transcription at PAX5-activated intergenic repeat (PAIR) elements located in the distal region of the Igh locus was completely dependent on ERG. These findings identify ERG as a novel critical regulator of B-cell development by ensuring efficient and balanced V-to-DJ recombination.
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| Overall design |
Microarray-based gene expression of FACS-sorted progenitor B-cells (Lin-, B220+, CD19+, CD93+ CD43+/lo) derived from Erg fl/fl (WT) and Erg fl/fl;CD2iCre (KO) mice. Three biological replicates were made for each genotype.
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| Contributor(s) |
Porse BT, Søndergaard E, Rauch A, Michaut M, Rapin N, Rehn M, Anna W, Camponeschi A, Hasemann MS, Bagger FO, Jendholm J, Knudsen KJ, Mandrup S, Mårtensson I |
| Citation(s) |
31775043 |
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| Submission date |
Mar 28, 2019 |
| Last update date |
Feb 23, 2020 |
| Contact name |
Bo Porse |
| Organization name |
University of Copenhagen
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| Department |
Finsen Laboratory and BRIC
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| Lab |
Porse lab
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| Street address |
Ole Maaløes Vej 5, 4
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| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE128978 |
ERG controls B-cell development by promoting Igh V-to-DJ recombination |
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| Relations |
| BioProject |
PRJNA529612 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE128976_RAW.tar |
22.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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