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Series GSE128976 Query DataSets for GSE128976
Status Public on Nov 24, 2019
Title ERG controls B-cell development by promoting Igh V-to-DJ recombination [microarray]
Organism Mus musculus
Experiment type Expression profiling by array
Summary B-cell development is dependent on the coordinated expression and cooperation of several transcription factors. Here, we show that the transcription factor ETS Related Gene (ERG) is crucial for normal B-cell development, and that its deletion results in a substantial loss of bone marrow B-cell progenitors and peripheral B-cells as well as a skewing of splenic B-cell populations. We found that ERG-deficient B-lineage cells exhibited an early developmental block, at the pre-B cell stage, and proliferated less. The cells failed to express the immunoglobulin heavy chain due to inefficient V-to-DJ recombination and cells that managed to undergo recombination displayed a strong bias against incorporation of distal V gene segments. Furthermore, antisense transcription at PAX5-activated intergenic repeat (PAIR) elements located in the distal region of the Igh locus was completely dependent on ERG. These findings identify ERG as a novel critical regulator of B-cell development by ensuring efficient and balanced V-to-DJ recombination.
 
Overall design Microarray-based gene expression of FACS-sorted progenitor B-cells (Lin-, B220+, CD19+, CD93+ CD43+/lo) derived from Erg fl/fl (WT) and Erg fl/fl;CD2iCre (KO) mice. Three biological replicates were made for each genotype.
 
Contributor(s) Porse BT, Søndergaard E, Rauch A, Michaut M, Rapin N, Rehn M, Anna W, Camponeschi A, Hasemann MS, Bagger FO, Jendholm J, Knudsen KJ, Mandrup S, Mårtensson I
Citation(s) 31775043
Submission date Mar 28, 2019
Last update date Feb 23, 2020
Contact name Bo Porse
Organization name University of Copenhagen
Department Finsen Laboratory and BRIC
Lab Porse lab
Street address Ole Maaløes Vej 5, 4
City Copenhagen
ZIP/Postal code 2200
Country Denmark
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (6)
GSM3689459 Gene_expr_microarray_KO1
GSM3689460 Gene_expr_microarray_KO2
GSM3689461 Gene_expr_microarray_KO3
This SubSeries is part of SuperSeries:
GSE128978 ERG controls B-cell development by promoting Igh V-to-DJ recombination
Relations
BioProject PRJNA529612

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128976_RAW.tar 22.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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