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Status |
Public on Jul 08, 2020 |
Title |
RNA sequencing of wildtype and PHOSPHO1 knockout brown fat |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We report the RNA expression of the mature brown fat from 6 week old wild type (WT) and PHOSPHO1 knockout (KO) mice. Mature brown fat was isolated from brown adipose tissue after collagenase digestion. Increased expression of mitochondrial genes is found in KO brown fat.
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Overall design |
Examine gene expression of two type of cells (WT and KO). Each sample is from 3 mice and each type of cells has triplicates (WT1-3 and KO1-3).
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Contributor(s) |
Huang N, Jiang M, Lodish H |
Citation(s) |
32554489 |
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Submission date |
Mar 29, 2019 |
Last update date |
Jul 08, 2020 |
Contact name |
Nai-Jia Huang |
E-mail(s) |
njhuang@wi.mit.edu
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Organization name |
Whitehead Institute
|
Lab |
Harvey Lodish
|
Street address |
455 Main Street
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City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA529783 |
SRA |
SRP189846 |