|
| Status |
Public on Oct 11, 2019 |
| Title |
Comparison of chromatin accessibility between human and non-human primates |
| Organisms |
Macaca mulatta; Gorilla gorilla; Pan troglodytes; Pongo pygmaeus; Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Evolution of transcriptional regulation is thought to be a major cause of the evolution of phenotypic traits. We compared DNase I Hypersensitive sites in fibroblast cells from five primates (human, chimpanzee, gorilla, orangutan, and macaque). We identified approximately 90,000 DHS sites, of which 59% are not significantly different between species, 27% are differential and likely due to a single evolutionary change, and 14% are differential and likely due to multiple changes. We found that including additional closely related species allows us to better distinguish between accessibility changes that are specific to a single species and those that have experienced changes in chromatin accessibility across multiple species during evolution.
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| |
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| Overall design |
DNase-seq of 3 biological replicates from each of 5 species: human, chimpanzee, gorilla, orangutan, and macaque.
Grant ID: HOMINID 0827552
Funding source: NSF
Grant title: Genetic Bases for the Evolution of Human Diet
Grantee name: Gregory Wray
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| |
|
| Contributor(s) |
Crawford G, Wray G |
| Citation(s) |
31599933 |
| |
| Submission date |
Mar 29, 2019 |
| Last update date |
Oct 21, 2019 |
| Contact name |
Gregory Crawford |
| E-mail(s) |
greg.crawford@duke.edu
|
| Organization name |
Duke University
|
| Street address |
101 Science Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platforms (7)
|
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL9160 |
Illumina Genome Analyzer II (Macaca mulatta) |
| GPL9378 |
Illumina Genome Analyzer II (Pan troglodytes) |
| GPL13766 |
Illumina Genome Analyzer IIx (Macaca mulatta) |
| GPL13802 |
Illumina Genome Analyzer IIx (Pan troglodytes) |
| GPL21650 |
Illumina HiSeq 2000 (Gorilla gorilla) |
| GPL26367 |
Illumina HiSeq 2000 (Pongo pygmaeus) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA529812 |
| SRA |
SRP189858 |
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