|
| Status |
Public on Aug 22, 2019 |
| Title |
RAG-mediated DNA breaks attenuate PU.1 activity in early B cells through activation of a SPIC-BCLAF1 complex [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
RAG DSBs trigger ATM-dependent induction of SPIC, an ETS family transcriptional repressor with significant homology to PU.1. RAG DSB-mediated expression of SPIC results in displacement of PU.1 throughout the genome and induction of broad transcriptional changes in pre B cells. SPIC also binds and recruits a unique partner, BCLAF1 to gene regulatory elements that regulate expression of key B cell developmental genes.
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| |
|
| Overall design |
ChIP was performed using anti-PU.1, anti-HA, and anti-BCLAF1
|
| |
|
| Contributor(s) |
Bednarski JJ |
| Citation(s) |
31644907 |
| BioProject |
PRJNA527987 |
| |
| Submission date |
Apr 01, 2019 |
| Last update date |
Nov 25, 2019 |
| Contact name |
Jeffery J Bednarski |
| E-mail(s) |
bednarski_j@wustl.edu
|
| Phone |
(314) 286-2825
|
| Organization name |
Washington University School of Medicine
|
| Department |
Department of Pediatrics
|
| Lab |
Bednarski Lab
|
| Street address |
660 S. Euclid Ave., Campus Box 8208
|
| City |
St. Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (11)
|
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| This SubSeries is part of SuperSeries: |
| GSE129130 |
RAG-mediated DNA breaks attenuate PU.1 activity in early B cells through activation of a SPIC-BCLAF1 complex |
|
| Relations |
| SRA |
SRP188818 |
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