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| Status |
Public on Sep 14, 2009 |
| Title |
Induction of the IL-6/signal transducer and activator of transcription pathway in the lungs of mice exposed to tobacco |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have examined the impact of subchronic levels of tobacoo smoke on murine lungs. We have used high density DNA microarray and analysed lung tissues from mice exposed to mainstream tobacco smoke (MTS). Our results reveal induction of inflammatory cytokine interleukin-6 (IL-6) and its antagonist suppressor of cytokine signalling (SOCS3) mRNA in smoking group. We also show that these changes are transient and are reversed completely after a period of cessation of smoking.
Keywords: Toxicology, disease state analysis, biomarkers of health effects
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| Overall design |
Male C57B1/CBA mice were exposed to MTS from two cigarettes daily, 5 days/week for 6 or 12 weeks. Mice were sacrificed immediately, or six weeks following the last cigarette. Left lung lobes were removed and flash frozen. Total RNA was isolated from a small part of the frozen lung and was hybridized against universal mouse reference RNA to Agilent Oligo DNA microarrays (Agilent Technologies) containing 22,000 transcripts. Microarrays were normalized using a global LOWESS approach and analyzed by MAANOVA 2.0 and SAM. Microarray results were validated by real time RT-PCR. Impact of alteration in expression of select genes was further validated by analysing their total protein levels in lung tissue homogenates.
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| Contributor(s) |
Halappanavar S, Russell M, Stampfli M, Williams A, Yauk CL |
| Citation(s) |
19698101 |
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| Submission date |
Sep 25, 2008 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Andrew Williams |
| Organization name |
Health Canada
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| Street address |
50 Columbine
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A OK9 |
| Country |
Canada |
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| Platforms (1) |
| GPL2881 |
Agilent-013326 Mouse Oligo Microarray (V2) G4121B (Feature Number version) |
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| Samples (39)
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| Relations |
| BioProject |
PRJNA110851 |
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