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| Status |
Public on Feb 15, 2011 |
| Title |
Auxin stimulates brassinosteroid biosynthesis in Arabidopsis roots |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We found that auxin stimulates gene expression of DWF4, which encodes a rate-dertermining step in brassinosteroid biosynthesis pathways. This increased gene expressioin subsequently led to elevation of the biosynthetic flux in Arabidopsis roots.
To determine the list of genes that are regulated by auxin-synthesizing brassinosteroids, we challenged Arabidopsis seedlings with either auxin only or auxin plus brassinosteroid biosynthetic inhibitor brassinazole.
Keywords: Hormone treatment
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| Overall design |
Arabidopsis seedlings (Columbia ecotype) were grown for 10 d on 1× MS agar-solidified media under long-day conditions (16:8, white light and dark cycle). The seedlings were then transferred to 2 different liquid media containing either 10–7 M 2,4-D or 10–7 M 2,4-D plus 10–6 M brassinazole. After 8 h of treatment, the seedlings were blotted with paper towels to remove excess media and subject to total RNA isolation. Total RNAs isolated from each batch were prepared from 3 replicate seedlings using an RNeasy plant mini kit (Qiagen, Germany).
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| Contributor(s) |
Choe S |
| Citation(s) |
21284753 |
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| Submission date |
Sep 29, 2008 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Sunghwa Choe |
| E-mail(s) |
shchoe@snu.ac.kr
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| Organization name |
Seoul National University
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| Department |
Biological sciences
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| Lab |
Biochemical genomics
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| Street address |
Gwanak-Gu Shillim-Dong
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| City |
Seoul |
| ZIP/Postal code |
151-747 |
| Country |
South Korea |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM325126 |
auxin-treated seedlings, biological rep1 |
| GSM325127 |
auxin-treated seedlings, biological rep2 |
| GSM325128 |
auxin-treated seedlings, biological rep3 |
| GSM325129 |
auxin and brassinazole-treated seedlings, biological rep1 |
| GSM325130 |
auxin and brassinazole-treated seedlings, biological rep2 |
| GSM325131 |
auxin and brassinazole-treated seedlings, biological rep3 |
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| Relations |
| BioProject |
PRJNA110955 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12964_RAW.tar |
13.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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