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| Status |
Public on Mar 05, 2020 |
| Title |
sRNA-seq of podocytes treated with glomerular endothelial cell vesicles |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The effect of miRNA delivery from glomerular endothelial cells (GEnCs) to podocytes in vitro was examined by miRNA epression in podocytes untreated and treated with extracellular vesicles from GEnCs under varied activation conditions.
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| Overall design |
Small RNA sequencing of primary human podocytes untreated (UT) or treated with extracellular vesicles (EVs) derived from human glomerular endothelial cells (GEnCs) for 24 hours. EVs were isolated by ultracentrifugation from supernatants of GEnCs in a steady state (SS) or activated with glucose (Glu) or puromycin aminonucleoside (PAN) for 24 hours.
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| |
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| Contributor(s) |
Hill N, Michell DL, Pusey C, Vickers KC, Woollard KJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 16, 2019 |
| Last update date |
Mar 05, 2020 |
| Contact name |
Danielle L Michell |
| E-mail(s) |
danielle.michell@vumc.org
|
| Organization name |
Vanderbilt Univ. Medical Center
|
| Department |
Department of Medicine
|
| Lab |
312 Preston Research Bldg
|
| Street address |
2220 Pierece Ave
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE129892 |
Glomerular endothelial derived vesicles mediate podocyte dysfunction via extracellular transfer of miRNA-200c-3p |
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| Relations |
| BioProject |
PRJNA533099 |
| SRA |
SRP192736 |
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