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Series GSE130109 Query DataSets for GSE130109
Status Public on Jul 26, 2019
Title miRNA profiling of blood samples from MWCNT exposed mice
Platform organisms Macaca mulatta; Homo sapiens; Mus musculus; Rattus norvegicus
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Pulmonary exposure to multiwalled carbon nanotubes (MWCNT) induces an inflammatory and rapid fibrotic response, although the long-term signaling mechanisms are unknown. The aim of this study was to examine the effects of 1, 10, 40, or 80 μg MWCNT administered by pharyngeal aspiration on bronchoalveolar lavage (BAL) fluid for polymorphonuclear cell (PMN) infiltration, lactate dehydrogenase (LDH) activity, and lung histopathology for inflammatory and fibrotic responses in mouse lungs 1 mo, 6 mo, and 1 yr postexposure. Further, a 120-μg crocidolite asbestos group was incorporated as a positive control for comparative purposes. Results showed that MWCNT increased BAL fluid LDH activity and PMN infiltration in a dose-dependent manner at all three postexposure times. Asbestos exposure elevated LDH activity at all 3 postexposure times and PMN infiltration at 1 mo and 6 mo postexposure. Pathological changes in the lung, the presence of MWCNT or asbestos, and fibrosis were noted at 40 and 80 μg MWCNT and in asbestos-exposed mice at 1 yr postexposure.
To identify non-invasive miRNA biomarkers, miRNA profiling was performed in blood samples collected from MWCNT exposed mice.
 
Overall design MWCNT used in this study were obtained from Hodogaya Chemical Company (MWNT-7, lot #05072001K28) and manufactured using a floating reactant catalytic chemical vapor deposition method followed by high temperature thermal treatment in argon at 2500°C using a continuous furnace (Kim et al. 2005). Extensive characterization of the MWCNT was previously published (Porter et al., 2010). In summary, the number of walls ranged from 20 to 50, median length was 3.86 ± 1.9 μm, and count mean width was 49 ± 13.4 nm. Trace metal contamination was 0.78%, with sodium (Na, 0.41%) and iron (Fe, 0.32%) being the two major metal contaminants. The crocidolite asbestos [(Na2(FeIII)2(FeII)3Si8O22(OH2)] used is this study was originally obtained from a mine in the Kalahari Desert, South Africa (Cheng et al., 1999) and previously characterized. Briefly, asbestos had a median fiber length of 11.5 μm (range: 2 – 30 μm), diameter of 160 – 800 nm, surface area of 17.1 m2/g, and Fe content (% weight) of 18% (Cheng et al., 1999, Shvedova et al., 2014). Endotoxin levels in the MWCNT and asbestos samples were below the levels of detection (Cheng et al., 1999; Porter et al., 2010). Male C57BL/J6 mice (7 weeks old, average weight 21.19 g ± 0.06 g) were obtained from Jackson Laboratories (Bar Harbor, ME). This study consisted of 3 components: bronchoalveolar lavage (BAL), pulmonary histopathology, and lung tissue mRNA microarray analysis. Each component consisted of 8 mice per exposure group (dispersion media [DM, negative control]; 1, 10, 40, or 80 μg MWCNT; 120 μg crocidolite asbestos [positive control]) to be sacrificed at 1, 6 or 12 months post-exposure, for a total of 144 mice per each arm of BAL, pulmonary histopathology, and lung tissue/blood miRNA microarray analysis and 432 mice for the total study.
 
Contributor(s) Guo NL
Citation(s) 31146342
Submission date Apr 21, 2019
Last update date Jul 26, 2019
Contact name Nancy Lan Guo
E-mail(s) lguo@hsc.wvu.edu
Phone 3042936455
Organization name West Virginia University
Department Occupational and Environmental Health Sciences
Lab Mary Babb Randolph Cancer Center/Guo Laboratory
Street address 2816 Health Sciences Center
City Morgatown
State/province WV
ZIP/Postal code 26506-9300
Country USA
 
Platforms (1)
GPL26525 ORB miRBase 19 Multispecies MicroRNA Microarray
Samples (144)
GSM3732092 136A_550_wb 51
GSM3732093 136B_550_wb 113
GSM3732094 136C_550_wb 216
This SubSeries is part of SuperSeries:
GSE131123 miRNA profiling of MWCNT exposed mice
Relations
BioProject PRJNA533976

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130109_2013-09-24_19723136_550PMT_5um_0532.txt.gz 593.1 Kb (ftp)(http) TXT
GSE130109_2013-09-24_19723137_550PMT_5um_0532.txt.gz 586.3 Kb (ftp)(http) TXT
GSE130109_2013-09-24_19723138_550PMT_5um_0532.txt.gz 591.6 Kb (ftp)(http) TXT
GSE130109_2013-09-24_19723139_550PMT_5um_0532.txt.gz 589.8 Kb (ftp)(http) TXT
GSE130109_2013-09-24_19723141_550PMT_5um_0532.txt.gz 590.9 Kb (ftp)(http) TXT
GSE130109_2013-09-24_19723142_550PMT_5um_0532.txt.gz 581.5 Kb (ftp)(http) TXT
GSE130109_2013-09-25_19723143_550PMT_5um_0532.txt.gz 577.5 Kb (ftp)(http) TXT
GSE130109_2013-09-25_19723144_550PMT_5um_0532.txt.gz 583.5 Kb (ftp)(http) TXT
GSE130109_2013-09-25_19723146_550PMT_5um_0532.txt.gz 579.1 Kb (ftp)(http) TXT
GSE130109_2013-09-25_19723147_550PMT_5um_0532.txt.gz 583.2 Kb (ftp)(http) TXT
GSE130109_2013-09-25_19723148_550PMT_5um_0532.txt.gz 588.8 Kb (ftp)(http) TXT
GSE130109_2013-09-25_19723149_550PMT_5um_0532.txt.gz 583.1 Kb (ftp)(http) TXT
GSE130109_2013-09-26_19723150_550PMT_5um_0532.txt.gz 594.0 Kb (ftp)(http) TXT
GSE130109_2013-09-26_19723151_550PMT_5um_0532.txt.gz 585.1 Kb (ftp)(http) TXT
GSE130109_2013-09-26_19723152_550PMT_5um_0532.txt.gz 589.5 Kb (ftp)(http) TXT
GSE130109_2013-09-26_19723153_550PMT_5um_0532.txt.gz 565.0 Kb (ftp)(http) TXT
GSE130109_2013-09-26_19723153_650PMT_5um_0532.txt.gz 346.3 Kb (ftp)(http) TXT
GSE130109_2013-09-26_19723154_550PMT_5um_0532.txt.gz 588.9 Kb (ftp)(http) TXT
GSE130109_2013-09-26_19723156_550PMT_5um_0532.txt.gz 593.1 Kb (ftp)(http) TXT
GSE130109_2013-09-27_19723157_550PMT_5um_0532.txt.gz 585.9 Kb (ftp)(http) TXT
GSE130109_2013-09-27_19723158_550PMT_5um_0532.txt.gz 583.8 Kb (ftp)(http) TXT
GSE130109_2013-09-27_19723159_550PMT_5um_0532.txt.gz 702.0 Kb (ftp)(http) TXT
GSE130109_2013-09-27_19723160_550PMT_5um_0532.txt.gz 587.5 Kb (ftp)(http) TXT
GSE130109_2013-09-27_19723161_550PMT_5um_0532.txt.gz 588.6 Kb (ftp)(http) TXT
GSE130109_2013-09-27_19723162_550PMT_5um_0532.txt.gz 599.8 Kb (ftp)(http) TXT
GSE130109_2013-09-27_19723162_550PMT_5um_redip_0532.txt.gz 332.4 Kb (ftp)(http) TXT
GSE130109_2013-10-01_19723163_550PMT_5um_0532.txt.gz 582.1 Kb (ftp)(http) TXT
GSE130109_2013-10-01_19723164_550PMT_5um_0532.txt.gz 600.4 Kb (ftp)(http) TXT
GSE130109_2013-10-01_19723165_550PMT_5um_0532.txt.gz 577.0 Kb (ftp)(http) TXT
GSE130109_2013-10-01_19723166_550PMT_5um_0532.txt.gz 580.1 Kb (ftp)(http) TXT
GSE130109_2013-10-01_19723167_550PMT_5um_0532.txt.gz 577.3 Kb (ftp)(http) TXT
GSE130109_2013-10-01_19723168_550PMT_5um_0532.txt.gz 592.0 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723169_550PMT_5um_0532.txt.gz 588.4 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723170_550PMT_5um_0532.txt.gz 593.9 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723170_550PMT_5um_redip_0532.txt.gz 328.3 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723171_550PMT_5um_0532.txt.gz 581.0 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723172_550PMT_5um_0532.txt.gz 586.3 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723172_550PMT_5um_redip_0532.txt.gz 330.6 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723173_550PMT_5um_0532.txt.gz 582.4 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723174_550PMT_5um_0532.txt.gz 592.8 Kb (ftp)(http) TXT
GSE130109_2013-10-02_19723174_550PMT_5um_redip_0532.txt.gz 331.5 Kb (ftp)(http) TXT
GSE130109_2013-10-03_19723175_550PMT_5um_0532.txt.gz 585.4 Kb (ftp)(http) TXT
GSE130109_2013-10-03_19723176_550PMT_5um_0532.txt.gz 332.3 Kb (ftp)(http) TXT
Processed data included within Sample table
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