 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 30, 2019 |
| Title |
Single-cell sequencing of Klinefelter testicular biopsies |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have evaluated the transcriptome of different testicular cell types, including germ cells, at single cell level in a testicular biopsy of a man with Klinefelter syndrome (XXY karyotype)
|
| |
|
| Overall design |
A testicular biopsy was enzymatically digested and single-cell RNAseq libraries were obtained using 10X genomics platform (10x Genomics Chromium Single Cell Reagent Kit v2). The libraries were sequenced on a NovaSeq600 sequencer (Illumina) using 2x150 bp paired-end sequencing.
|
| |
|
| Contributor(s) |
Laurentino S, Heckmann L, Di Persio S, Li X, Meyer zu Hörste G, Wistuba J, Cremers J, Gromoll J, Kliesch S, Schlatt S, Neuhaus N |
| Citation(s) |
31462300 |
| Submission date |
Apr 22, 2019 |
| Last update date |
Sep 02, 2019 |
| Contact name |
Sandra Laurentino |
| Organization name |
University of Münster
|
| Street address |
Domagkstr. 11
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA534098 |
| SRA |
SRP193364 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE130151_RAW.tar |
13.0 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
