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| Status |
Public on May 03, 2019 |
| Title |
Expression data comparing CD133+ and CD133- B16-F10 melanoma cells |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Cancer stem cells (CSC) were isolated based on the putative stem cell marker CD133. This subset of cells has been shown to have superior tumorigenicity and metastatic ability compared to cells in the non-CSC compartment (i.e. CD133-). We compared microRNA expression profiles of CSCs to non-CSCs to identify disparities in miRNA expression and explore how these miRNAs may provide a selective survival advantage to cancer stem cells.
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| Overall design |
B16-F10 murine melanoma cells were labelled with a monocloncal antibody against CD133 (Prominin-1) and subjected to FACS to collect CD133-negative and -positive populations arising from the parental cell population. Total RNA was isolated and hybridized on Affymetrix GeneChips (miRNA 4.0 platform). Chips were scanned using a GeneChip Scanner (Affymetrix) and microarray images were analyzed using Affymetrix Power Tools. Transcriptome Analysis Software (TAC, Affymetrix) was used to analyze and visualize microarray data.
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| Contributor(s) |
Shidal C, Singh N, Nagarkatti P, Nagarkatti M |
| Citation(s) |
31015227 |
| Submission date |
Apr 24, 2019 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Chris P Shidal |
| E-mail(s) |
chris.shidal@vumc.org
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| Phone |
6158752761
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| Organization name |
Vanderbilt University Medical Center
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| Department |
Medicine
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| Lab |
Qiuyin Cai
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| Street address |
2525 West End Ave 6th Floor
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
27203 |
| Country |
USA |
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| Platforms (1) |
| GPL21572 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA534522 |
