 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 09, 2008 |
| Title |
Global profiling of B. subtilis comparing wild-type to mutants with altered cytoplasmic membrane composition |
| Organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by array
|
| Summary |
The Bacillus subtilis membrane contains diacylglycerol-based lipids with at least five distinct headgroups that impart distinct physical and chemical properties to the lipid bilayer. Here, we describe the phenotypic characterization of mutant strains lacking one or more of the following lipids: glycolipids (ugtP mutants), phosphatidylethanolamine (pssA and psd mutants), lysylphosphatidylglycerol (mprF), and cardiolipin (ywnE and ywjE). Alterations of membrane lipid headgroup composition are generally well tolerated by the cell and even severe alterations lead to only modest effects on growth proficiency. Mutants with decreased levels of positively charged lipids display an increased sensitivity to a cationic antimicrobial compounds and cells lacking glycolipids are sensitive to the lantibiotic sublancin and are defective in swarming motility. A quadruple mutant strain (ugtP pssA mprF ywnE), with a membrane comprised predominantly of phosphatidylglycerol, is viable and grows at near wild-type rates, although it forms long, coiled filaments. Transcriptome comparisons identify numerous regulons with altered expression in ugtP mutant cells, the pssA mprF ywnE triple mutant, and the uptP pssA mprF ywnE quadruple mutant. These effects include a general decrease in expression of the SigD and FapR regulons, and increased expression of cell envelope stress responses mediated by ?M and the YvrGHb two-component system.
|
| |
|
| Overall design |
WT vs.ugtP single mutant (U), WT vs. mprFpssAywnE triple mutant (T) or WT vs. ugtPmprFpssAywnE quadruple mutant (Q). Each experiment (WT vs. mutant) was conducted four times using three independent total RNA preparations (3 independent experiement + dye swap). For the 3 datasets used for final analysis wild-type was labeled with Alexa Fluor 555 and mutants with Alexa Fluor 647. For dye swap experiments wild-type was labeled with Alexa Fluor 647 and mutants with Alexa Fluor 555.
|
| |
|
| Contributor(s) |
Salzberg LI, Helmann JD |
| Citation(s) |
18820022 |
| |
| Submission date |
Oct 03, 2008 |
| Last update date |
Feb 20, 2014 |
| Contact name |
John D. Helmann |
| E-mail(s) |
jdh9@cornell.edu
|
| Phone |
607 255 6570
|
| Organization name |
Cornell University
|
| Department |
Microbiology
|
| Street address |
372 Wing Hall
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7420 |
Sigma Genosys Panorama Bacillus subtilis Gene Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA109709 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13036_RAW.tar |
14.9 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...