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| Status |
Public on May 07, 2019 |
| Title |
RNA-seq data for Arabidopsis cbfs-1 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In Arabidopsis, CBFs transcription factors (CBF1, CBF2 and CBF3) play fundamental roles in plant cold tolerance, especially in cold-acclimation. By employing CRISPR/Cas9, we knocked out CBF1 and CBF2 simultaneously in cbf3 background and generated cbfs triple mutant. This mutant facilitated the discovery of CBF-regulated genes. In this study, Arabidopsis 14-d-old Col-0 and cbfs mutant were treated with cold stress (4 degree centigrade) for 0, 3 and 24 hours. The seedlings were harvested for total RNA extraction and sequencing.
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| Overall design |
14-day-old Col and cbfs-1 seedlings were treated under 4 degree for 0, 3 and 24 h. The RNA were extracted for RNA-sequencing.
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| Contributor(s) |
Jia Y, Yang S |
| Citation(s) |
27353960 |
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| Submission date |
May 06, 2019 |
| Last update date |
May 07, 2019 |
| Contact name |
Yuxin Jia |
| Organization name |
China Agricultural University
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| Department |
Biology
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| Street address |
No. 2 Yuanmingyuan west road, Haidian district
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| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA541229 |
| SRA |
SRP195488 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE130729_YSH.deseq_stdgtf.DESeq2.normalized.txt.gz |
1.9 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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