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| Status |
Public on May 31, 2021 |
| Title |
Effect of Cell Division on Cell Differentiation (RNA-Seq H9 cells UD E24 E24HU E24Noc) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We addressed the role of cell division during differentiation by blocking the progression of the cell cycle during mesendoderm differentiation. Human pluripotent stem cells (H9 cells) were synchronized in their cell cycle and then induced to differentiate into mesendoderm for 24h. To block the cells in their S phase, we added Hydroxyurea (200 uM) at the beginning of the differentiation process. To block the cells in their G2/M phases, we added nocodazole (100 ng/ml) 8h after the induction of differentiation. At the end of the experiment, the cells were harvested and processed for RNA extraction.
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| Overall design |
Four different conditions were analyzed: Undifferentiated (UD), Mesendoderm (E24), Mesendoderm + Hydroxyurea (E24HU), and Mesendoderm + nocodazole (E24Noc). Two biological replicates were generated for each condition.
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| Contributor(s) |
Grandy R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 07, 2019 |
| Last update date |
Jun 01, 2021 |
| Contact name |
Rodrigo Andres Grandy |
| Organization name |
University of Cambridge
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| Department |
Surgery
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| Lab |
Vallier Lab
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| Street address |
Cambridge Stem Cell Institute/ Anne McLaren Laboratory West Forvie Building, Robinson Way
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0SZ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE130830 |
Effect of Cell Division on Cell Differentiation |
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| Relations |
| BioProject |
PRJNA541536 |
| SRA |
SRP195819 |
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