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| Status |
Public on Feb 24, 2020 |
| Title |
small RNA NGS based diagnostic survey of Hungarian grapevine rootstock plantations |
| Organism |
Vitis vinifera |
| Experiment type |
Other
|
| Summary |
Several pathogens infect grapevine, including viruses and viroids. Considering that there are no effective plant protection treatments against these pathogens and vineyards are cultivated through decades usage of high quality and pathogen-free propagation material (rootstocks and scions) is essential.
Although presence of regulated pests is routinely checked using ELISA or rarely RT-PCR, these diagnostics methods can detect only particular pathogens moreover can fail to detect variant strains. High-throughput sequencing of small RNAs can be an effective, alternative method to avoid these disadvantages. Since for production of grafts, pathogen free cultivars and rootstocks must be used, 17 grapevine rootstock plantations and 2 rootstock variety collections were selected for characterisation of their virom by high throughput sequencing of virus derived small RNAs.
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| Overall design |
Virus diagnotics of 17 Hungrarian grapevine rootstock plantation and 2 grapevine rootstock variety collection by Illumina sequencing of the small RNAs
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| Contributor(s) |
Demián E, Jaksa-Czotter N, Molnár J, Tusnády GE, Kocsis L, Várallyay É |
| Citation(s) |
34200935 |
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Demian E, Jaksa-Czotter N, Molnar J, Tusnady GE, Laszlo K, and Varallyay E. Grapevine rootstocks can be a source of infection with non-regulated viruses. Eur J Plant Pathol (2020). doi:10.1007/s10658-020-01942-w
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| Submission date |
May 10, 2019 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Éva Várallyay |
| E-mail(s) |
varallyay.eva@abc.naik.hu
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| Organization name |
National Agricultural Research and Innovation Centre
|
| Department |
Agricultural Biotechnology Institute
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| Lab |
Diagnostics
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| Street address |
4 Szent-Györgyi Albert Street
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| City |
Gödöllő |
| ZIP/Postal code |
2100 |
| Country |
Hungary |
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| Platforms (1) |
| GPL18740 |
Illumina HiSeq 2000 (Vitis vinifera) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA542259 |
| SRA |
SRP197522 |
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