 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 09, 2020 |
| Title |
Transcriptomic profile of bpm2,3,5 triple mutant and Col-0 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The Jasmonate pathway regulators MYC2, MYC3 and MYC4 are central nodes in plant signaling networks integrating environmental and developmental signals to fine-tune jasmonate defenses and plant growth. Hence, their activity needs to be tightly regulated in order to optimize plant fitness. Among the increasing number of mechanisms regulating MYCs activity, protein stability is arising as a major player. However, how the levels of MYCs proteins are modulated is still poorly understood. Here, we report that MYC2, MYC3 and MYC4 are targets of BPM proteins, which act as substrate adaptors of CUL3-based E3 ubiquitin ligases. Reduction-of-function of CUL3BPM in amiR-bpm lines, bpm235 triple mutants and cul3ab double mutants enhances MYC2 and MYC3 stability and accumulation, and potentiates plant responses to JA such as root-growth inhibition, and MYC-regulated gene expression. BPM3 protein is stabilized by JA, suggesting a new negative feed-back regulatory mechanism to control MYCs activity. Our results uncover a new layer for JA-pathway regulation by CUL3BPM–mediated degradation of MYC TFs.
|
| |
|
| Overall design |
Comparison of gene expression of bpm2,3,5 triple mutant versus Col-0
|
| |
|
| Contributor(s) |
Chico JM, Fernandez-Barbero G, García-Casado G, Franco-Zorrilla JM, Solano R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 10, 2019 |
| Last update date |
Feb 19, 2020 |
| Contact name |
Jose M. Franco-Zorrilla |
| Organization name |
Centro Nacinal de Biotecnologia-CSIC
|
| Department |
Plant Molecular Genetics
|
| Street address |
Darwin, 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL22511 |
Agilent-045560 Arabidopsis thaliana 8x60k v1_complete |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA542339 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE131024_RAW.tar |
105.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...