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| Status |
Public on May 01, 2009 |
| Title |
Mutator transposon activity alters the transcriptome and proteome of developing maize anthers |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
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| Summary |
MuDR/Mu are a highly active transposon family moving by either cut only (or cut-and-paste) in strictly somatic tissues or net replicative transposition (absence of excision alleles) in reproductive tissues. Aside from the
MuDR-encoded MURA and MURB proteins, other factors required for Mu transposition, particularly those contributing to the developmentally specific behavior, have yet to be identified. To address this question and assess
the impact of a highly active transposon on the transcriptome, RNA was extracted from anthers at three developmental stages in Mu-active and -inactive stocks and compared on a 44,000 element oligonucleotide array.
We found that approximately 30,000 unique genes are expressed at each stage. Of the ~10% (ca. 3000) differentially regulated transcripts per stage, there was approximately equal representation by the active or inactive individuals.
Keywords: anther development, maize, Mu, transposon
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| Overall design |
Dye swap design with 4 replicates comparing Mu-active to -inactive lines at 3 anther stages. Spike-in controls were included.
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| Contributor(s) |
Skibbe DS, Walbot V, Fernandes JF |
| Citation(s) |
24939185 |
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| Submission date |
Oct 08, 2008 |
| Last update date |
Jun 30, 2014 |
| Contact name |
John F Fernandes |
| E-mail(s) |
jfernand@stanford.edu
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| Phone |
650-533-9376
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| Organization name |
Stanford University
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| Department |
Bio Sci
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| Lab |
Walbot
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| Street address |
385 Serra Mall
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL7444 |
Agilent-016047 Walbot Zea mays 4x44K array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA109655 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13118_RAW.tar |
371.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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