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| Status |
Public on Jul 10, 2019 |
| Title |
Genome-wide identification of binding sites and gene targets of Alx1, a pivotal regulator of echinoderm skeletogenesis |
| Organism |
Strongylocentrotus purpuratus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ChIP-seq provides a genome-wide view of the binding sites of Alx1, a pivotal transcription factor in a gene regulatory network that controls skeletogenesis in sea urchins and other echinoderms.
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| Overall design |
Three independent cultures of S. purpuratus were collected and processed at the mesenchyme blastula stage (24 hpf). For each culture, ChIP was performed using α-Sp-Alx1 and normal rabbit IgG (Sigma-Aldrich, Cat. No. 12370) was used for mock IP.
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| Contributor(s) |
Khor J, Ettensohn CA |
| Citation(s) |
31331943 |
| |
| Submission date |
May 16, 2019 |
| Last update date |
Oct 09, 2019 |
| Contact name |
Jian Ming Khor |
| E-mail(s) |
jkhor@andrew.cmu.edu
|
| Organization name |
Carnegie Mellon University
|
| Department |
Biological Sciences
|
| Lab |
Ettensohn
|
| Street address |
4400 Fifth Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL20965 |
Illumina HiSeq 2500 (Strongylocentrotus purpuratus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA543356 |
| SRA |
SRP198689 |
