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| Status |
Public on Dec 10, 2019 |
| Title |
BRCA1 mutation influences progesterone response in human benign mammary organoids. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mammary organoids treated with a 28 day menstrual cycle profile of estradiol and progesterone with or without TPA: Comparison of normal versus BRCA1 mut cells. Purpose: The goals of this study are to determine 1) the effects of a 28 day menstrual cycle profile of estradiol and progesterone, 2) the effects of TPA, and 3) the responses in normal versus BRCA1 carriers, in benign breast organoids using RNA-seq
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| Overall design |
Breast organoids from patients without (normal) or with BRCA1 mutations were cultured in vitro. Organoids from each patient were divided into 2 groups. One group was treated with estradiol and progesterone and the second group was treated with estradiol, progesterone and TPA. Hormone treatment over the course of 28 days was as follows: 0.1nM estradiol on days 1-7, 1nM estradiol on days 7-14, 1nM estradiol + 10nM progesterone (with or without 1uM TPA) on days 14-21 and 0.1nM estradiol + 50nM progesterone (with or without 1uM TPA) on days 21-28. Media was changed every 2 days.
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| Contributor(s) |
Kim JJ, Davaadelger B |
| Citation(s) |
31771627 |
| |
| Submission date |
May 22, 2019 |
| Last update date |
Dec 10, 2019 |
| Contact name |
Julie Kim |
| E-mail(s) |
j-kim4@northwestern.edu
|
| Phone |
312-503-5377
|
| Organization name |
Northwestern University
|
| Department |
Obstetrics and Gynecology
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| Lab |
Lurie 4-250
|
| Street address |
303 E. Superior St
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA544257 |
| SRA |
SRP199204 |
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