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Series GSE131640 Query DataSets for GSE131640
Status Public on Dec 10, 2019
Title BRCA1 mutation influences progesterone response in human benign mammary organoids.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Mammary organoids treated with a 28 day menstrual cycle profile of estradiol and progesterone with or without TPA: Comparison of normal versus BRCA1 mut cells. Purpose: The goals of this study are to determine 1) the effects of a 28 day menstrual cycle profile of estradiol and progesterone, 2) the effects of TPA, and 3) the responses in normal versus BRCA1 carriers, in benign breast organoids using RNA-seq
Overall design Breast organoids from patients without (normal) or with BRCA1 mutations were cultured in vitro. Organoids from each patient were divided into 2 groups. One group was treated with estradiol and progesterone and the second group was treated with estradiol, progesterone and TPA. Hormone treatment over the course of 28 days was as follows: 0.1nM estradiol on days 1-7, 1nM estradiol on days 7-14, 1nM estradiol + 10nM progesterone (with or without 1uM TPA) on days 14-21 and 0.1nM estradiol + 50nM progesterone (with or without 1uM TPA) on days 21-28. Media was changed every 2 days.
Contributor(s) Kim JJ, Davaadelger B
Citation(s) 31771627
Submission date May 22, 2019
Last update date Dec 10, 2019
Contact name Julie Kim
Phone 312-503-5377
Organization name Northwestern University
Department Obstetrics and Gynecology
Lab Lurie 4-250
Street address 303 E. Superior St
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (24)
GSM3791603 B1 BRCA E+P
GSM3791604 B2 BRCA E+P
GSM3791605 B3 BRCA E+P
BioProject PRJNA544257
SRA SRP199204

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131640_RAW.tar 2.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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