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Series GSE13168 Query DataSets for GSE13168
Status Public on Nov 01, 2008
Title Effects of glucocorticoids and Protein Kinase A on growth factor- and 1beta- regulated gene
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Glucocorticoids (GCs) and protein kinase A (PKA)-activating agents (beta-adrenergic receptor agonists) are mainstream asthma therapies based on their ability to prevent or reverse excessive airway smooth muscle (ASM) constriction. Their abilities to regulate another important feature of asthma - excessive ASM growth are poorly understood. Recent studies have suggested that GCs render agents of inflammation such as interleukin 1beta and tumor necrosis factor alpha mitogenic to ASM, via suppression of (antimitogenic) induced cyclooxygenase-2-dependent PKA activity. To further explore the mechanistic basis of these observations, we assessed the effects of epidermal growth factor and interleukin 1beta stimulation, and the modulatory effects of GC treatment and PKA inhibition, on the ASM transcriptome by microarray analysis.

Keywords: gene expression
 
Overall design Human ASM cultures were generated from trachea of 4 unidentified donors and used in passage 5-8. These cells were used to generate cells stabely expressing a PKA inhibitor (PKI) or GFP (control), which were then grown to confluence, serum starved for 24h, and stimulated with IL-1b, EGF,or both (E+I) in presence or absence of Fluticasone (Flu, 30 min pretreatment). After 8h stimulation cells were washed and total RNA isolated using TRIzol as per manufacturer's protocol. Gene expression was analyzed in 4 different cultures derived from 4 different donors (for a total of 3-4 microarrays per condition).
 
Contributor(s) Misior AM, Penn RB
Citation(s)
  • Misior AM, Deshpande DA, Loza MJ, Pascual RM et al. Glucocorticoid- and protein kinase A-dependent transcriptome regulation in airway smooth muscle. Am J Respir Cell Mol Biol 2009 Jul;41(1):24-39. PMID: 19059887
Submission date Oct 13, 2008
Last update date Aug 10, 2018
Contact name Anna Misior
Organization name Wake Forest University
Department Center for Human Genomics
Lab Penn Laboratory
Street address Medical Center Blvd
City Winston-Salem
State/province NC
ZIP/Postal code 27157
Country USA
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (54)
GSM332913 GFP_basal_donor1
GSM332914 GFP_basal_donor2
GSM332915 GFP_basal_donor3
Relations
BioProject PRJNA109637

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13168_RAW.tar 112.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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