|
| Status |
Public on Mar 17, 2020 |
| Title |
Gene expression profiling of Tsp1 deficient rat aortic smooth muscle cells with mechanical stretch |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The extracellular matrix (ECM) initiates mechanical cues and transduces intracellular signaling through matrix-cell interactions. The nature of cues and how they coordinate with a mechanical microenvironment are not fully understood. We identified the matricellular protein, thrombospondin-1 (Tsp1, also called Thbs1), as a mediator of matrix mechanotransduction that acts via integrin αvβ1 to establish focal adhesions and promotes nuclear shuttling of Yes-associated protein (YAP) in response to cyclic stretch. Thbs1-mediated YAP activation depends on the small GTPase Rap2 and Hippo pathway, and is not influenced by altered actin fibers. Hence, to gain insight into the molecular mechanisms underlying the Tsp1-mediated matrix mechanotransduction, we performed a comprehensive analysis of gene expression changes in Tsp1deficiant SMCs with mechanical stretch using RNA sequencing (RNA-Seq).
|
| |
|
| Overall design |
mRNA profiles of rat aortic smooth muscle cells were generated by RNA sequencing using the NextSeq 500 (Illumina).
|
| |
|
| Contributor(s) |
Yamashiro Y, Kim J |
| Citation(s) |
32321834 |
| Submission date |
May 24, 2019 |
| Last update date |
Jun 16, 2020 |
| Contact name |
Jun-Dal Kim |
| Organization name |
University of Toyama
|
| Department |
Department of Complex Biosystem Research (CBR), Institute of National Medicine (INM)
|
| Lab |
Complex Biosystem Research Lab.
|
| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA544677 |
| SRA |
SRP199435 |
Less...
More...