|
| Status |
Public on Feb 21, 2009 |
| Title |
The protein kinase Tor1 regulates adhesin expression in Candida albicans |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by array
|
| Summary |
Eukaryotic cell growth is coordinated in response to nutrient availability, growth factors, and environmental stimuli, enabling cell–cell interactions that promote survival. The rapamycin-sensitive Tor1 protein kinase, which is conserved from yeasts to humans, participates in a signaling pathway central to cellular nutrient responses. To gain insight into Tor-mediated processes in human fungal pathogens, we have characterized Tor signaling in Candida albicans. Global transcriptional profiling revealed evolutionarily conserved roles for Tor1 in regulating the expression of genes involved in nitrogen starvation responses and ribosome biogenesis. Interestingly, we found that in C. albicans Tor1 plays a novel role in regulating the expression of several cell wall and hyphal specific genes, including adhesins and their transcriptional repressors Nrg1 and Tup1. In accord with this transcriptional profile, rapamycin induced extensive cellular aggregation in an adhesin-dependent fashion. Moreover, adhesin gene induction and cellular aggregation of rapamycin-treated cells were strongly dependent on the transactivators Bcr1 and Efg1. These findings support models in which Tor1 negatively controls cellular adhesion by governing the activities of Bcr1 and Efg1. Taken together, these results provide evidence that Tor1-mediated cellular adhesion might be broadly conserved among eukaryotic organisms.
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| Overall design |
Study consists of wild type Candida albicans cells grown in Spider liquid medium at 35C and treated either with vehicle or 20 nM rapamycin. Analysis is based off 4 independent biological replicates
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| Contributor(s) |
Bastidas RJ, Heitman J, Cardenas ME |
| Citation(s) |
19197361 |
| |
| Submission date |
Oct 13, 2008 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Robert J Bastidas |
| E-mail(s) |
rjb10@duke.edu
|
| Phone |
919-684-2809
|
| Organization name |
Duke University
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Cardenas/Heitman Lab
|
| Street address |
322 CARL, Research Drive Box 3546
|
| City |
Durham |
| State/province |
N.C |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7476 |
OPERON_AROSV1.1.2_Candida_albicans_array |
|
| Samples (12)
|
| GSM333015 |
Candida albicans cells in Spider medium upon treatment with the Tor1 inhibitor rapamycin (R1). |
| GSM333016 |
Candida albicans cells in Spider medium upon treatment with the Tor1 inhibitor rapamycin (R2). |
| GSM333017 |
Candida albicans cells in Spider medium upon treatment with the Tor1 inhibitor rapamycin (R3). |
| GSM333018 |
Candida albicans cells in Spider medium upon treatment with the Tor1 inhibitor rapamycin (R4). |
| GSM333019 |
Candida albicans cells in YPD medium upon treatment with the Tor1 inhibitor rapamycin (R1). |
| GSM333020 |
Candida albicans cells in YPD medium upon treatment with the Tor1 inhibitor rapamycin (R2). |
| GSM333021 |
Candida albicans cells in YPD medium upon treatment with the Tor1 inhibitor rapamycin (R3). |
| GSM333022 |
Candida albicans cells in YPD medium upon treatment with the Tor1 inhibitor rapamycin (R4). |
| GSM333023 |
C. albicans TOR1-1/TOR1 cells in YPD medium upon treatment with the Tor1 inhibitor rapamycin (R1). |
| GSM333024 |
C. albicans TOR1-1/TOR1 cells in YPD medium upon treatment with the Tor1 inhibitor rapamycin (R2). |
| GSM333025 |
C. albicans TOR1-1/TOR1 cells in YPD medium upon treatment with the Tor1 inhibitor rapamycin (R3). |
| GSM333026 |
C. albicans TOR1-1/TOR1 cells in YPD medium upon treatment with the Tor1 inhibitor rapamycin (R4). |
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| Relations |
| BioProject |
PRJNA109719 |
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