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| Status |
Public on Jan 28, 2006 |
| Title |
Hypoxic response in wild type and HIF-1alpha null hepatoctyes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The primary aim of this study was to evaluate the changes in hepatocyte gene expression under short-term hypoxic conditions in wild type and HIF-1a null cultures. To this end, hypoxia treated cultures were subjected to incubation with 1% O2/5% CO2/94% N2 at 37° C for eight hours prior to RNA isolation. Duplicate normoxic controls were established from separate animals wherein cultures were untreated and treated with Adbgal. Biological triplicates of wild type and HIF-1a null cultures were placed under hypoxic conditions and subsequently processed for microarray analysis. A total of 10 microarray hybridizations were performed.
Keywords: repeat sample
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| Contributor(s) |
Allen JW, Khetani SR, Johnson RS, Bhatia SN |
| Citation(s) |
17518628 |
| Submission date |
Apr 20, 2004 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Jared Allen |
| E-mail(s) |
jallen@bioeng.ucsd.edu
|
| Phone |
858-822-3128
|
| URL |
http://mtel.ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Department |
Bioengineering
|
| Lab |
Microscale Tissue Engineering Laboratory
|
| Street address |
9500 Gilman Drive, 0412
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
|
| Samples (10)
|
| GSM21757 |
Normoxic Adeno Treated Control N2 |
| GSM21760 |
Hypoxia Adeno treated sample N1 |
| GSM21762 |
Hypoxia Adeno Treated Sample N2 |
| GSM21764 |
Hypoxia Adeno Treated Sample N3 |
| GSM21766 |
Hypoxia HIF-1alpha null sample N1 |
| GSM21768 |
Hypoxia HIF-1alpha null sample N2 |
| GSM21770 |
Hypoxia HIF-1alpha null sample N3 |
|
| Relations |
| BioProject |
PRJNA89895 |
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