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| Status |
Public on Dec 22, 2020 |
| Title |
H3K9me3 ChIP-seq in MLL-rearranged leukemic stem cells and hematopoietic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Acute myeloid leukemia (AML) with rearrangement of the mixed-lineage leukemia (MLL) gene are the most aggressive hematopoietic malignancies. Previous studies demonstrated the distribution of several epigenetic modifications including H3K9me3, H3K79me2, H3K36me3, H3K4me3 and H3K27me3, in MLL-AF9 transformed murine cells. Here, we examined the H3K9me3 distribution in c-Kit+ cells (enriched with stem/progenitor cells) from both MLL-AF9 transformed murine cells in parallel with control wild-type cells, and found an overall lower distribution of H3K9me3 in leukemia stem cells than normal hematopoietic stem/progenitor cells.
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| Overall design |
c-Kit+ cells were isolated with CD117 beads from bone marrow of wild-type and moribund leukemic mice (MLL-AF9 and MLL-NRIP3 fusion transformed murine c-Kit+ cells). c-Kit+ cells were then cross-linked, sonicated and incubated with H3K9me3 antibody. ChIPed DNA were purified with classic phenol chloroform extraction protocol. H3K9m3 ChIPed DNA profiles of these c-Kit+ cells were generated by DNA sequencing using Illumina Hiseq2500.
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| Contributor(s) |
Yajing C |
| Citation(s) |
33037410 |
| |
| Submission date |
Jun 04, 2019 |
| Last update date |
Dec 22, 2020 |
| Contact name |
Yajing Chu |
| E-mail(s) |
chuyajing@ihcams.ac.cn
|
| Phone |
86-22-23909396
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| Organization name |
Institute of Hematology, Chinese Academy of Medical Sciences
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| Department |
State Key Laboratory of Experimental Hematology
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| Lab |
Weiping Yuan Lab
|
| Street address |
Nanjing Road 288
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| City |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA546260 |
| SRA |
SRP200401 |
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