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| Status |
Public on Jun 12, 2019 |
| Title |
Gene expression analysis of Mtb-infected mouse alveolar macrophages from mice lacking Nrf2 in LysM- or CD11c-expressing cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim of this study was to measure the immune response of alveolar macrophages (AMs) from mice lacking Nrf2 in LysM- or CD11c-expressing cells to intracellular Mtb infection in vivo. We characterized the transcriptional profile of murine Mtb-infected AMs after aerosol infection by sorting cells from bronchoalveolar lavage fluid and performing low-input RNA-sequencing.
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| Overall design |
To characterize the early macrophage transcriptional response to Mtb infection, we infected mice with 2000-4000 CFU of mEmerald-H37Rv, and isolated AMs (CD45+, Zombie Violet-, CD3-/CD19-, Siglec-F+, CD11bmid, and CD64+) from BAL by fluorescence activated cell sorting at 24 hours following infection. Three populations were sorted for analysis by RNA-seq: Mtb-infected AMs (mEmerald+), bystander AMs (mEmerald-), and AMs from naïve mice.
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| Contributor(s) |
Rothchild AC, Diercks AH, Aderem A |
| Citation(s) |
31350281 |
| |
| Submission date |
Jun 04, 2019 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Aderem Lab |
| E-mail(s) |
alan.diercks@seattlechildrens.org
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| Organization name |
Seattle Children's Research Institute
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| Lab |
Aderem Lab
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| Street address |
307 Westlake Avenue North, Suite 500
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA546272 |
| SRA |
SRP200413 |
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