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| Status |
Public on Sep 02, 2020 |
| Title |
MicroRNA transcriptome profiling in ovarian tissues of pigs with extreme phenotypes for reproductive efficiency during the luteal and follicular phases of the estrous cycle |
| Organism |
Sus scrofa |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose:The goal of this study was to enrich understanding of the reproductive difference
Methods: Multiparous Canadian Large White cyclic sows were divided into two parts: high (H; total number of piglets born > 15.73) and low (L; total number of piglets born < 11.11) fecundity. Eight sows with similar parity from each part were chosen (n=16). Ovarian tissues were obtained on the 14 day (day 1 = first day of estrus) after estrus as in the luteal phase (L) and 20 day of the estrous cycle as in the follicular phase (F). Transcriptome profiling of ovarian tissues were generated by deep sequencing, in quadruplicate, using Illumina HiSeq X10 instrument.
Results: Using an optimized data analysis workflow, we obtained the differentially expressed miRNAs between the high and low fecundity, with a fold change ≥1.5 and p value < 0.05. These results provide further insight into fecundity in pigs.
Conclusions: Our study represents the first detailed analysis of ovary transcriptome. It will be significantly helpful to display a novel regulatory mechanism for further investigation of prolificacy in pigs.
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| Overall design |
Eight sows with similar parity from high and low fecundity group were chosen, respectively. Ovarian tissues were obtained on the 14 day (day 1 = first day of estrus) after estrus as in the luteal phase (L) and 20 day of the estrous cycle as in the follicular phase (F). Transcriptome profiling of ovarian tissues were generated by deep sequencing, in quadruplicate, using Illumina HiSeq X10 instrument.
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| Contributor(s) |
Hu H, Jia Q |
| Citation(s) |
32928107 |
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| Submission date |
Jun 06, 2019 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Huiyan HU |
| E-mail(s) |
huhuiyan315@163.com
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| Phone |
+86-312-7528343
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| Organization name |
Hebei Agricultural University
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| Street address |
Lekai South Street No. 2596
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| City |
Baoding |
| ZIP/Postal code |
071000 |
| Country |
China |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE134002 |
Profiling in ovarian tissues of pigs with extreme phenotypes for reproductive efficiency during the luteal and follicular phases of the estrous cycle |
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| Relations |
| BioProject |
PRJNA547580 |
| SRA |
SRP200659 |
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