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| Status |
Public on Aug 22, 2019 |
| Title |
miRNA expression profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME) |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We report the miRNA profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME) after supplementation with polyunsaturated fatty acids (PUFA) and stimulation with LPS/pro-inflammatory cytokines. The fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6) were included in the culture medium in concentrations of 15 µmol/L using ethanol as a vehicle (0.2 % v/v final ethanol concentration). Cells were cultured in the enriched media totaling either 72 h (RAW264.7) or 144 h (TIME). Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of either LPS (1 µg/mL; from E. coli serotype 0111:B4) for cell line RAW264.7 or the cytokines IL-1β, TNF-α, and IFN-γ each in a concentration of 5 ng/ml for cell line TIME.
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| Overall design |
miRNA profiles of macrophages (cell line: RAW264.7) and endothelial cells (cell line: TIME) supplemented with unsaturated fatty acids and exposed to an inflammatory milieu were generated by deep sequencing, in triplicate, using Illumina HiScanSQ.
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| Contributor(s) |
Schumann J, Roessler C, Krohn K |
| Citation(s) |
31484960, 33003296, 34831066, 35205256, 37301912 |
| |
| Submission date |
Jun 07, 2019 |
| Last update date |
Sep 08, 2023 |
| Contact name |
Julia Schumann |
| E-mail(s) |
julia.schumann@uk-halle.de
|
| Organization name |
University Medicine Halle (Saale)
|
| Street address |
Franzosenweg 1a
|
| City |
Halle (Saale) |
| ZIP/Postal code |
06112 |
| Country |
Germany |
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| Platforms (2) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
| GPL16173 |
Illumina HiScanSQ (Mus musculus) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA547723 |
| SRA |
SRP200732 |
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