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Status |
Public on Feb 18, 2020 |
Title |
High-throughput RNA sequencing analysis of human colon cancer cell line HCT116 transfected with LINC00152 siRNA. |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to evaluate the effects of si-LINC00152 on the mRNA of human colon cancer cell line HCT116. Methods: Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA to cells or transfected with siRNA targeting LINC00152 for 36 hours in DMEM medium (with 10% serum). Total RNA were extracted and detected by Illumina high-throughput RNA sequencing data analysis. 2 independent biological replicates were plated, transfected in parallel for each control and siRNA. Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. There are 90 mRNAs were up-regulated and 159 were down-regulated in “si-LINC00152” group comparing to “control” group. Conclusions: Our study describes the mRNA changes of human colon cancer cell line HCT116 transfected with LINC00152 siRNA.
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Overall design |
Human colon cancer cell line HCT116 mRNA profiles of “control” (cells was transfected with a control non-targeting siRNA) and“si-LINC00152”(cells was transfected with LINC00152 siRNA) were generated by RNA sequecning, using Solexa high-throughput sequencing service (Oebiotech, Shanghai, China).
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Contributor(s) |
Ou C, Ma J |
Citation(s) |
32042551 |
Submission date |
Jun 11, 2019 |
Last update date |
Apr 20, 2022 |
Contact name |
Jian Ma |
E-mail(s) |
majian@csu.edu.cn
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Organization name |
Central South University
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Department |
Cancer Research Institute
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Street address |
110 Xiangya Road
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City |
Changsha |
State/province |
Hunan |
ZIP/Postal code |
410078 |
Country |
China |
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Platforms (1) |
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Samples (4)
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GSM3872544 |
Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA, biological rep 1 |
GSM3872545 |
Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA, biological rep 2 |
GSM3872546 |
Human colon cancer cell line HCT116 was transfected with LINC00152 siRNA, biological rep 1 |
GSM3872547 |
Human colon cancer cell line HCT116 was transfected with LINC00152 siRNA, biological rep 2 |
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Relations |
BioProject |
PRJNA548279 |
SRA |
SRP201071 |