|
| Status |
Public on Jun 03, 2020 |
| Title |
Expression profiling of mouse HPC7 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have expressed mouse Snai1, mutant Snai1 or empty vector in the HPC7 cell line and performed RNA sequencing analysis to identify targets of Snai1 in mouse hematopoietic progenitor cells.
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| |
|
| Overall design |
HPC7 cells were engineered to express either wildtype Snai1, a mutant version of Snai1 that cannot bind Lsd1 or an empty vector control using retroviral transduction. GFP+ cells were sorted after 1 week in culture and RNA was extracted for RNA sequencing.
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| |
|
| Contributor(s) |
Carmichael CL, Ritchie ME, Haigh JJ |
| Citation(s) |
32369597 |
| |
| Submission date |
Jun 13, 2019 |
| Last update date |
Jun 03, 2020 |
| Contact name |
Matthew Ritchie |
| E-mail(s) |
mritchie@wehi.edu.au
|
| Organization name |
The Walter and Eliza Hall Institute of Medical Research
|
| Department |
Epigenetics and Development Division
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA548811 |
| SRA |
SRP201394 |
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