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| Status |
Public on Jun 19, 2022 |
| Title |
Lactobacillus brevis strains UCCLBBS124 and UCCLBBS449, control (MRS broth) vs. stress condition (5 % EtOH, pH4 or 30 ppm iso-a-acids) |
| Organism |
Levilactobacillus brevis |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptional profiling of Lactobacillus brevis UCCLBBS124 and UCCLBBS449 comparing control strain grown in MRS broth with strains growing in different stress conditons (5 % EtOH, pH4 or 30 ppm iso-a-acids).This study aimed to evaluate how certain Lb. brevis isolates are adapted so as to allow them to survive and grow in beer.
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| Overall design |
DNA-microarrays containing oligonucleotide primers combining shared and unique genes of the 2648 and 2719 annotated genes on the genome of Lb. brevis UCCLBBS124 and UCCLBBS449, respectively, were designed by and obtained from Agilent Technologies (Palo Alto, Ca., USA). Three diffrerent experiments: MRS broth vs. MRS broth 5% EtOh, MRS broth vs. MRS broth pH4, MRS broth vs. MRS broth 30 ppm iso-a-acids for each of the two Lb. brevis strains UCCLBBS124 and UCCLBBS449
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| Contributor(s) |
Feyereisen M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 20, 2019 |
| Last update date |
Jun 20, 2022 |
| Contact name |
Marine Feyereisen |
| E-mail(s) |
116221209@umail.ucc.ie
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| Organization name |
Univeristy College Cork
|
| Street address |
Western raod
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| City |
Cork |
| State/province |
Cork |
| ZIP/Postal code |
T12 YT20 |
| Country |
Ireland |
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| Platforms (1) |
| GPL26808 |
Agilent-084674 Lactobacillus brevis microarray |
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| Samples (12)
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| GSM3899304 |
UCCLBBS124 MRS broth vs MRS broth 5% EtOH |
| GSM3899305 |
UCCLBBS124 MRS broth vs MRS broth 5% EtOH Dye swap |
| GSM3899306 |
UCCLBBS124 MRS broth vs MRS broth pH4 |
| GSM3899307 |
UCCLBBS124 MRS broth vs MRS broth pH4 Dye swap |
| GSM3899308 |
UCCLBBS124 MRS broth vs MRS broth 30 ppm iso-a-acids |
| GSM3899309 |
UCCLBBS124 MRS broth vs MRS broth 30 ppm iso-a-acids Dye swap |
| GSM3899310 |
UCCLBBS449 MRS broth vs MRS broth 5% EtOH |
| GSM3899311 |
UCCLBBS449 MRS broth vs MRS broth 5% EtOH Dye swap |
| GSM3899312 |
UCCLBBS449 MRS broth vs MRS broth pH4 |
| GSM3899313 |
UCCLBBS449 MRS broth vs MRS broth pH4 Dye swap |
| GSM3899314 |
UCCLBBS449 MRS broth vs MRS broth 30 ppm iso-a-acids |
| GSM3899315 |
UCCLBBS449 MRS broth vs MRS broth 30 ppm iso-a-acids Dye swap |
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| Relations |
| BioProject |
PRJNA549870 |
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