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| Status |
Public on Dec 15, 2008 |
| Title |
Expression, ChIP-chip, and ChIP-Seq data from REH and SEM leukemia cell lines [Expression and ChIP-chip] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
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| Summary |
MLL-fusion proteins are potent inducers of cancer in hematopoietic cells, where they are known to cause changes in global gene expression. How MLL-fusion proteins interact with the genome has not been established, so we have limited understanding of the pathway by which these proteins generate aberrant gene expression programs. Here we describe how the MLL-AF4 protein occupies the genome in human leukemia cells and its striking effects on chromatin states. We find that the MLL-AF4 fusion protein selectively occupies regions of the genome that contain developmental regulatory genes important for hematopoietic stem cell identity and self-renewal. These MLL-AF4 bound regions have grossly altered chromatin structure, with histone modifications catalyzed by Trithorax Group (TrxG) proteins and Dot1 extending across unusually large domains. This indicates that a key feature of MLL-associated leukemogenesis is aberrant targeting of chromatin modifiers to regions of the genome controlling hematopoietic development. Our results define the direct targets of the MLL-fusion protein, reveal the global role of epigenetic misregulation in leukemia, and identify new targets for therapeutic intervention in human cancer.
Keywords: cell type comparison
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| Overall design |
This dataset includes expression data for two replicates each of SEM and REH leukemia cell lines and ChIP-chip data targeting RNAP2, H3K4me3, H3K79me2, ENL, AF4-C, and MLL-N in SEM and REH leukemia cell lines.
Related ChIP-Seq data are provided in GEO Series GSE38338.
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| Contributor(s) |
Frampton GM |
| Citation(s) |
19141473 |
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| Submission date |
Oct 23, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Garrett M Frampton |
| E-mail(s) |
frampton@mit.edu
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| Organization name |
Whitehead Instute - MIT
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| Department |
Biology
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| Lab |
Richard Young
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| Street address |
9 Cambridge Center
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (2) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
| GPL7804 |
Agilent 95k Human Promoter v1 013590 |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA109897 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13313_RAW.tar |
115.4 Mb |
(http)(custom) |
TAR (of CEL, GPR) |
| Processed data included within Sample table |
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