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| Status |
Public on Apr 16, 2020 |
| Title |
CARD9-associated Dectin-1 and Dectin-2 are Required for Protective Immunity of a Multivalent Vaccine Against Coccidioides posadasii Infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Preparation of sequencing libraries and sequencing analysis using Illumina HiSeq 3000 platform (50 bp single-read module) were conducted at the Genomics Core of UT Health San Antonio. Sequencing reads were preprocessed by Trim Sequences tools in CLC Genomics workbench. Remaining reads were then aligned to mouse GRCm38(mm10) reference genome using RNA-Seq Analysis module in CLC Genomics workbench. To determine differentially expressed genes, exact test were performed after filtering lowly expressed gene (counts per million < 1 in more than three samples across the dataset) using edgeR package (Robinson, McCarthy et al. 2010). Gene Ontology (GO) analysis were performed using DAVID (Dennis, Sherman et al. 2003) and then visualized by GOplot (Walter, Sanchez-Cabo et al. 2015).
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| Overall design |
BMMs were incubated with GCP-rCpa1 at a ratio of 50:1 for 12 hours with 5% CO2. BMMs incubated with PBS served as controls. Total RNA was isolated from BMMs cells using the Qiagen RNeasy kit.
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| Web link |
https://www.jimmunol.org/content/204/12/3296.long
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| |
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| Contributor(s) |
Hung C, Zhang H, Campuzano A |
| Citation(s) |
32358020 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI135005 |
Development of a multivalent vaccine against Coccidioides infection |
THE UNIVERSITY OF TEXAS AT SAN ANTONIO |
Chiung Yu Hung |
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| |
| Submission date |
Jun 21, 2019 |
| Last update date |
Jul 16, 2020 |
| Contact name |
Chiung-Yu Hung |
| Organization name |
The University of Texas at San Antonio
|
| Department |
Biology
|
| Lab |
MBT 1.308
|
| Street address |
One UTSA Circle
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78249 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA550079 |
| SRA |
SRP202204 |
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